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- 文献和实验
- 技术资料
- 英文名：
Biotin-PEG3-Maleimide
- 供应商：
靶点科技
- 规格：
25 mg
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文献和实验Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition
of maleimide reporter groups to 5??diene?modified oligonucleotides via aqueous Diels?Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene?amidite (5??diene modifier), for the attachment of a diene group
were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
Site-directed hydroxy radical mapping of nucleosome positions in vitro
for site directed mapping is BABE (bromoacetamidobenzyl EDTA (Meares et al ., 1984)). However, the predominant side reaction of common thiol-active functional groups such as bromoacetamide and maleimide is with lysine. There are a very large number
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