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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
IAA | Auxin ELISA quantitation kit
- CAS号:
/
- 保质期:
详见说明
- 供应商:
齐源生物
- 保存条件:
2-8℃
上海齐源生物科技有限公司长期提供各种生物生化试剂盒,ELISA试剂盒价格实惠,质量有保证,信誉第一。
齐源生物提供ELISA试剂盒代测,生化试剂盒代测,木质素代测,纤维素代测服务。
齐源生物是专业生产生化试剂盒的厂家,价格优惠,现货供应。
齐源生物代理AgriseraI,Agrisera的所有抗体分两大类:植物及藻类的抗体,以及细菌、真菌、昆虫、鱼等动物类抗体,植物领域的抗体涵盖Arabidopsis thaliana、Hordeum vulgare、Chlamydomonas reinhardtii、Cyanobacteria、Physcomitrella patens、Oryza sativa、Populus sp、Conifers等多个物种。
AA | 生长素ELISA定量试剂盒说明:
ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The IAA-indole-3-acetic acid (C1') hormone specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples.
Reaction wells : antibody coated and blocked, 5pcs for 480 assays, 60 strips with 8 wells
Tracer : 20 – 50 µl
Tracer diluent : 5 x 250 mM TBS Tris, 10 mM NaCl, 1 mM MgCl2, pH 7.5 stock + 0.02 % NaN3
Reaction and wash solution : 10x TBS stock+0.02 % NaN3
Stopping reagent : 2x 5 N KOH stock
Substrate diluent : 10x 500 mM NaHCO3 stock, pH 9.6+0 0.02 %. 0 0.02 % NaN3
Substrate : 100 mg
Standards : 600 µl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol, 15.2 fmol
Assay development time : 4-5 hours
Sensitivity : 0.01 to 10 pmol/50 µl
Plant extract volume : 50 µl (for using on algae - please inquire)
Cross reactivity to skatole is 3.3 %; this antibody will also bind IAA glucosylester.
Assay parameters
Unspecific binding : 2.5 %
Midrange(B/Bo=50%) : 0.02-10 pmol; Detection limit : 12.25 pg (7 fmol)
Linear range of measurment : 15-500 fmol; Intraassay variability : 3.6 %
Interassay variability : 4.3 %; Amount of tracer per assay : 10 ng
齐源生物科技有限公司长期现货出售各种进口代理品牌:瑞典Agrisera,美国USP、美国R&D、QIAGEN凯杰、BioVision、sigma西格玛、加拿大TRC、加拿大TLC、美国CATO、美国O2si、GIBCO、日本wako、PeproTech、默克Merck、MBL、Invitrogen、OXOID、Hyclone、CST、Bio-Rad、Bender Medsystems、美国BD、Agrisera、ABNOVA、ABI、ABCAM、Thermo、赛默飞thermofisher、BP(英国药典)、德国Dr、Anogen、EXBIO、Demmeditec、Cell Biolabs、Prolmmune、ScyTek等。
齐源生物代理Agrisera其它产品:
齐源生物提供ELISA试剂盒代测,生化试剂盒代测,木质素代测,纤维素代测服务。
齐源生物是专业生产生化试剂盒的厂家,价格优惠,现货供应。
齐源生物代理AgriseraI,Agrisera的所有抗体分两大类:植物及藻类的抗体,以及细菌、真菌、昆虫、鱼等动物类抗体,植物领域的抗体涵盖Arabidopsis thaliana、Hordeum vulgare、Chlamydomonas reinhardtii、Cyanobacteria、Physcomitrella patens、Oryza sativa、Populus sp、Conifers等多个物种。
AA | 生长素ELISA定量试剂盒说明:
ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The IAA-indole-3-acetic acid (C1') hormone specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples.
Reaction wells : antibody coated and blocked, 5pcs for 480 assays, 60 strips with 8 wells
Tracer : 20 – 50 µl
Tracer diluent : 5 x 250 mM TBS Tris, 10 mM NaCl, 1 mM MgCl2, pH 7.5 stock + 0.02 % NaN3
Reaction and wash solution : 10x TBS stock+0.02 % NaN3
Stopping reagent : 2x 5 N KOH stock
Substrate diluent : 10x 500 mM NaHCO3 stock, pH 9.6+0 0.02 %. 0 0.02 % NaN3
Substrate : 100 mg
Standards : 600 µl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol, 15.2 fmol
Assay development time : 4-5 hours
Sensitivity : 0.01 to 10 pmol/50 µl
Plant extract volume : 50 µl (for using on algae - please inquire)
Cross reactivity to skatole is 3.3 %; this antibody will also bind IAA glucosylester.
Assay parameters
Unspecific binding : 2.5 %
Midrange(B/Bo=50%) : 0.02-10 pmol; Detection limit : 12.25 pg (7 fmol)
Linear range of measurment : 15-500 fmol; Intraassay variability : 3.6 %
Interassay variability : 4.3 %; Amount of tracer per assay : 10 ng
齐源生物科技有限公司长期现货出售各种进口代理品牌:瑞典Agrisera,美国USP、美国R&D、QIAGEN凯杰、BioVision、sigma西格玛、加拿大TRC、加拿大TLC、美国CATO、美国O2si、GIBCO、日本wako、PeproTech、默克Merck、MBL、Invitrogen、OXOID、Hyclone、CST、Bio-Rad、Bender Medsystems、美国BD、Agrisera、ABNOVA、ABI、ABCAM、Thermo、赛默飞thermofisher、BP(英国药典)、德国Dr、Anogen、EXBIO、Demmeditec、Cell Biolabs、Prolmmune、ScyTek等。
齐源生物代理Agrisera其它产品:
| 标签抗体组(Cas9 / HA / LUC / C-YFP / N-YFP) | Tag Antibody Set(Cas9/HA/LUC/C-YFP/N-YFP) | AS17-4127 |
| 总黄qu霉毒素燛LISA定量试剂盒 | Total Aflatoxins ELISA quantitation kit | AS20-4395 |
| ZEA 犛衩壮嗝瓜┩狤LISA定量试剂盒 | ZEA Zearalenone ELISA quantitation kit | AS20-4393 |
| ABA 犕崖渌酔LISA定量试剂盒(5x96T) | ABA Abscisic acid ELISA quantitation kit (5x96T) | AS20-4392-5x96 |
| ABA 犕崖渌酔LISA定量试验试剂盒(24T) | ABA Abscisic acid ELISA quantitation trial kit (24T) | AS20-4392-24 |
| 2 光合作用工具套件的定量 | 2Photosynthesis Tool Kit-quantitation | AS04-051 |
| 2 组4种衣藻抗Lhc抗体 | 2Set of 4 Chlamydomonas anti-Lhc antibodies | AS01-011-CHLAMYDOMONAS |
| 2 教育抗体试剂盒-光合作用 | 2Educational antibody kit-photosynthesis | AS05-070 |
| 2 一组10种植物抗Lhca和抗Lhcb抗体 | 2Set of 10 plant anti-Lhca and anti-Lhcb antibodies | AS01-011 |
| 2 Rubisco定量试剂盒 | 2Rubisco quantitation kit | AS09-409 |
| ABA 脱落酸ELISA定量试剂盒 | ABAAbscisic acid ELISA quantitation kit | AS11-1748 |
| IAA Auxin ELISA定量试剂盒 | IAAAuxin ELISA quantitation kit | AS11-1749 |
| 反玉米蛋白核苷细胞分裂素酶联免疫吸附测定定量试剂盒 | Trans-zeatin ribosideCytokinin ELISA quantitation kit | AS11-1784 |
| 顺式玉米素核苷细胞分裂素酶联免疫吸附测定定量试剂盒 | Cis-zeatin ribosideCytokinin ELISA quantitation kit | AS12-1844 |
| N6-异戊烯基腺苷细胞分裂素ELISA定量试剂盒 | N6-isopentenyladenosineCytokinin ELISA quantitation kit | AS12-1845 |
| N6-苄基腺苷细胞分裂素ELISA定量试剂盒 | N6-benzyladenosineCytokinin ELISA quantitation kit | AS12-1846 |
| DNA损伤(8-OHdG)ELISA试剂盒 | DNA Damage(8-OHdG)ELISA kit | AS15-2887 |
| DNA损伤(8-OHdG)ELISA试剂盒,X5 | DNA Damage(8-OHdG)ELISA kit, X5 | AS15-2887X5 |
| Rubisco ELISA定量试剂盒 | Rubisco ELISA quantitation kit | AS15-2994 |
| 植物维生素A ELISA定量试剂盒 | Plant Vitamin A ELISA quantitation kit | AS15-3024 |
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文献和实验相关实验
植物吲哚乙酸(IAA) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定植物相关样本中吲哚乙酸(IAA)含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 植物吲哚乙酸(IAA ) 水平。用纯化的 植物吲哚乙酸(IAA ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 植物吲哚乙酸(IAA ) ,再与HRP 标记的 植物吲哚乙酸(IAA ) 抗体结合,形成抗体
IAA | 生长素ELISA定量试剂盒
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