Rat TCP11L2 shRNA Plasmid/Rat

产品名称：2 |光合作用工具套件的定量-2|Photosynthesis Tool Kit-quantitation

产品货号：AS04-051

产品规格：1kit

背景资料：This kit allows quantitation of major photosynthetic proteins: Rubisco, PsaC (PSI) and PsbA (PSII) as well as determination of PSI/PSII ratios using the western blot technique. The kit contains following global antibodies, protein standards and secondary antibody: Rabbit anti-RbcL (Form I and Form II). Product number: AS03 037 Rabbit anti-PsaC. Product number: AS10 939Rabbit anti-PsbA Product number: AS05 084Rubisco protein standard. Product number: AS01 017S PsaC protein standard. Product number: AS04 042S PsbA protein standard. Product number: AS01 016S Goat anti-Rabbit IgG (H&L), HRP conj. (50 ul), Product number: AS09 602

保存建议：2 |光合作用工具套件的定量-2|Photosynthesis Tool Kit-quantitationStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

产品描述：Product information - Primary antibodies:Product number:Product name:Reconstitution: Recommended dilution:AS03 037Rabbit Anti-RbcL Global antibodyFor reconstitution see lable on respective tube.1:5000-10 000 with standard ECLAS10 939Rabbit Anti-PsaC Global antibodyFor reconstitution see lable on respective tube.1:1000 with standard ECLAS05 084Rabbit Anti-PsbAGlobal antibodyFor reconstitution see lable on respective tube.1:10 000 with standard ECL* All primary antibodies in this kit are raised in rabbits.Product information - Protein standards:Product number:Product name:Concentration:Size:Western Blot ae Positive Control:AS01 016SPsbA *0.25 pmol/ μl41.5 kDa#To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.AS01 017SRbcL *0.15 pmol/ μl52.7 kDaTo generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range.A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.AS04 042SPsaC *0.15 pmol/μl11.5 kDa#To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 aul). For most applications a sample load of 0.2 aug of chlorophyll will give a PsaC signal in this range.A 2 ul load is optimal for most chemiluminescent detection systems as a positive control.*These proteins are larger than a respective native protein due to the addition of His-tag* For reconstitution of standards see lable on respective tube.Product information - Secondary antibody:AS09 602- Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 aul (2x25 aul)Educational information about Quantitative western blot can be found here: detailed method description, video tutorial

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关键词：细胞分析试剂盒，20453 |光合作用工具套件的定量，待更新