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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
收到产品后,建议在2度至8度的温度下存放多一年。
- 保质期:
详询艾美捷客服
- 英文名:
Anti-Fade Mounting Medium (With DAPI)
- 库存:
Anti-Fade Mounting Medium (With DAPI)
- 供应商:
艾美捷
- CAS号:
Anti-Fade Mounting Medium (With DAPI)
- 规格:
10ml
产品名称:Anti-Fade Mounting Medium (With DAPI)-Anti-Fade Mounting Medium (With DAPI)
产品货号:abx090690
产品规格:10ml
背景资料:待更新
保存建议:Anti-Fade Mounting Medium (With DAPI)-Anti-Fade Mounting Medium (With DAPI)收到产品后,建议在2度至8度的温度下存放多一年。
产品描述:Anti-Fade Mounting Medium (With DAPI) is a glycerol-based mounting medium containing an anti-fluorescence quenching reagent. It can be used to seal tissue or cell slides for fluorescence-related applications. After mounting, slides can be observed for several weeks without drying out or requiring sealing. This medium can help prolong fluorescence and maximise fluoresence intensity, and assist with antigen-antibody binding.
This medium is compatible with a wide range of fluorophores and fluorescent proteins, such as blue fluorescent protein, FITC, Cy2, Cy3, Cy5, Cy7, DAPI, rhodamine, Texas Red, AMCA, R6G, Hoechst 33258, AF, and more. This medium contains DAPI, a blue fluorescent DNA stain which binds strongly to "A-T"-rich regions in DNA, exhibiting up to a 20-fold increase in fluorescence. It can pass through intact cell membranes, and therefore can be used to stain both live and fixed cells for fluorescence microscopy and flow cytometry. It can also be used for counting cells, measuring apoptosis, sorting cells based on DNA content, and as a nuclear segmentation tool in imaging analysis. DAPI has an absorption maxima at 340 nm and an emission maxima at 488 nm. When bound to double-stranded DNA, the absorption maxima changes to 358 nm (ultraviolet) and the emission maxima to 461 nm (blue).点击:Anti-Fade Mounting Medium (With DAPI)-Anti-Fade Mounting Medium (With DAPI)查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-908垂询Abbexa中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品网站。
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关键词:生化试剂,Anti-Fade Mounting Medium (With DAPI),待更新
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文献和实验A semi-permanent mounting medium for immunofluorescence microscopy
months. The solution is stable at room temperature for 1 month. Small amounts of DABCO or other fluorescence stabilizer (anti-fade agent) added to each aliquot immediately before use will reduce signal fading under UV light.
Fluorescence Mounting Medium (Antifade)
should appear almost colorless to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer
PREPARATION OF ANTI-BLEACHING MOUNTING MEDIUM
PREPARATION OF ANTI-BLEACHING MOUNTING MEDIUM Materials 1. p-phenylenediamine, 1%(w/v) in distilled water, 1 ml. Adjust pH to 9.5 with NaOH. Use the highest grade from Sigma. 2. glycerol. 3. 10x simple PBS. Procedure
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