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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
收到产品后,建议储存在-20度多一年。
- 保质期:
详询艾美捷客服
- 英文名:
Protease, Phosphatase and PMSF
- 库存:
Protease, Phosphatase and PMSF
- 供应商:
艾美捷
- CAS号:
Protease, Phosphatase and PMSF
- 规格:
1ml
产品名称:Protease, Phosphatase and PMSF-Protease, Phosphatase and PMSF
产品货号:abx090639
产品规格:1ml
背景资料:待更新
保存建议:Protease, Phosphatase and PMSF-Protease, Phosphatase and PMSF收到产品后,建议储存在-20度多一年。
产品描述:Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and PMSF are used to prevent protein degradation during Western Blots.
Protease Inhibitor Cocktail is a mixture of 6 different protease inhibitors. This inhibits of a broad range of proteases and esterases, including aspartyl proteases, cysteine proteases, serine proteases, and aminopeptidases.
Phosphatase Inhibitor Cocktail is a mixture of 5 different phosphatase inhibitors. It inhibits many tyrosine phosphatases, acid phosphatases, and alkaline phosphatases.
PMSF (100 mM) is used for inhibiting serine proteases, including tryptase, chymotrypsin, callicrein, subtilisin, and thrombin. It also inhibits the cysteine protease papain.
This product is provided as three vials:- Protease Inhibitor Cocktail (1 ml, 100X)
- Phosphatase Inhibitor Cocktail (1 ml, 100X)
- PMSF (1 ml, 100 mM, 100X)
点击:Protease, Phosphatase and PMSF-Protease, Phosphatase and PMSF查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-886垂询Abbexa中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品网站。
Abbexa总部位于英国剑桥的剑桥科学园,是一家专业的全球抗体供应商和蛋白质供应商,致力于为生命科学、药物开发和生物技术领域的科学家和研究者提供佳的客户体验和高质量的产品。产品涵盖一抗、二抗、蛋白质、ELISA试剂盒、酶以及其他用于研究的试剂盒和工具。无论您是进行学术研究,药物开发,疾病研究还是其它生物学研究,Abbexa的单克隆抗体,多克隆抗体,蛋白质和生物试剂均旨在促进您的研究。如果很难找到合适的抗体来满足您的需求,Abbexa还提供了定制蛋白质,肽和抗体的生产渠道。
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关键词:生化试剂,Protease, Phosphatase and PMSF,待更新
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文献和实验检测蛋白磷酸化一定要加磷酸酶抑制剂!!除此以外还应该加蛋白酶抑制剂。 http://www.millipore.com/techpublications/tech1/mcproto402 Protease Inhibitors · EDTA: 1 mM · PMSF: 1 mM · Aprotinin, leupeptin, pepstatin: 1 µg/ml each Phosphatase Inhibitors ·
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
Buffer does not contain protease or phosphatase inhibitors. However, if desired, protease and phosphatase inhibitors can be added to the RIPA buffer just before use to prevent proteolysis and maintain phosphorylation of proteins.) PMSF
Preparation of Modifed Radioimmunoprecipitation (RIPA) Buffer
at 2-8� until ready to use. 5. Ideally, the remaining protease and phosphatase inhibitors should be added to the solution on the same day the assay is run (100 microliters of aprotinin, leupeptin, and pepstatin; 500 microliters of PMSF, Na3 VO
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