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文献和实验Flow Cytometry Assay to Measure Internalization of GPCRs
. However, the user can probably also substitute PBS Ca-free, Mg-free plus 0.04% EDTA to lift cells.7) Pellet cells in centrifuge set at 4degC and rinse 1x with media. NOTE: Since the anti-FLAG M1 antibody (Sigma, Covance)requires Ca ions to bind the FLAG epitope
Flow Cytometry Assay to Measure Internalization of GPCR's
PBS Ca-free, Mg-free plus 0.04% EDTA to lift cells. 7) Pellet cells in centrifuge set at 4degC and rinse 1x with media. NOTE: Since the anti-FLAG M1 antibody ( Sigma , Covance )requires Ca ions to bind the FLAG epitope, you must rinse
Flow Cytometry Assay to Measure Internalization of GPCR
cells. 7) Pellet cells in centrifuge set at 4degC and rinse 1x with media. NOTE: Since the anti-FLAG M1 antibody ( Sigma , Covance )requires Ca ions to bind the FLAG epitope, you must rinse the pellet with media to get rid of any residual
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