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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温保存
- 保质期:
见标签
- 库存:
现货
- 供应商:
鹿森生物
- CAS号:
9000-71-9
- 规格:
瓶装
应用
生化/生理作用
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文献和实验to about 10 ml/50-100 g. 10 min at 15900 g. Resuspend pellets in NN (lysis) buffer (4-5 ml/50-75 g). Add SDS to 0.5% (250 l of 10%/5 ml NN). Swirl thoroughly. Add proteinase K to 100 g/ml (25 l of 20 mg/ml/5 ml NN). Swirl gently. 60 min. 37 ℃. Add
at 15900 g. 13.Resuspend pellets in NN (lysis) buffer (4-5 ml/50-75 g). 14.Add SDS to 0.5% (250 l of 10%/5 ml NN). Swirl thoroughly. 15.Add proteinase K to 100 g/ml (25 l of 20 mg/ml/5 ml NN). Swirl gently. 16.60 min 37 ℃
Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 1 ml buffer 1.Be careful not to introduce bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight. 4.Collect actin
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