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- 技术资料
- 保存条件：
常温
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现货
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联硕生物
- 规格：
1瓶
一般描述
Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.[1]
应用
Asialofetuin from fetal calf serum has been used:
to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)[2]
quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA[3]
as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins[4]
to study the nature of the interaction between ferritin and the placenta[5]
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
生化/生理作用
Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R.
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文献和实验Studies on lithium acetate doped chitosan conducting polymer system
Yahya MZA and Arof AK
European Polymer Journal, 38(6), 1191-1197 (2002)
Serum-Resistant Lipoplexes in the Presence of Asialofetuin
“lipoplexes,” which offered some promise for an easy and efficient liposomal gene delivery. In this protocol, we describe the preparation of serum-resistant lipoplexes in the presence of the ligand asialofetuin (AF), in order to design efficient gene therapy
细胞系等，该细胞有一定自发转化倾向，一般在转染前一天接种细胞，接种密度为2×104 /cm2 ,用含10%胎牛血清的DMEM液，37℃、5%CO2 培养，待细胞占50～70%瓶底面积时，用于转染试验。 (3)DNA-磷酸钙沉淀物的制备 ①将供体细胞DNA和PSV2-neo质粒载体DNA用TE配制成40mg/L的DNA溶液，同时向供体细胞DNA液200μl中加入带基因neo质粒DNA液(20mg/L)220μl和2×HBS 250μl(PSV2-neo为1～2mg/L的使用量)。 ②取500μl上述
大，由hyclone公司总部的员工采集加工，采集方法，加工过程，检定标准与美国原产优等胎牛血清完全一致，市场价格低于美国原产的。经过3次100nm过滤，内毒素含量≤25EU/ml，血红蛋白含量≤25mg/dl 2、Hyclone的南美优级胎牛血清 大体与加拿大的优等血清是一样的，级别也非常相似，货号是sv30087.02，该胎牛血清来源于南美洲，以封闭的心脏穿刺方式采集，产品经3次100nm 过滤，经严格检测确保无支原体，细菌病毒检测依据美国药典(USP)和美国联邦法规第九篇(9CFR
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