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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric) (96 assays)
- 供应商:
上海西宝
- 规格:
96次分析
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文献和实验has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity in small,snap frozen pieces of tissue (e.g.,colon polyps),or in cultured cells. 1.Homogenize Cells/Tissues For cells in culture: Harvest
DMB (DNMT-Magnetic Beads) Assay: Measuring DNA Methyltransferase Activity In Vitro
a novel assay system, in which the activity of a DNA methyltransferase is measured as the incorporation of tritium into biotinylated DNA oligonucleotides. The DNA is immobilized onto magnetic beads with streptavidin covalently attached to the bead surface
Quantitate proteins in 15 ml aliquots of lysates (in duplicate) by using a Bradford assay (I have been using the Biorad kit), using BSA as standard. Don't forget to add 15 ml LB to each BSA sample and to the blank tube. Use the same LB batch as that used
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