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- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
EpiQuik Tri-Methyl-Histone H3K9 ChIP Kit (24 reactions)
- 规格:
24次反应
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
dilution). Multiple ChIP reactions can be performed from one 3C ligation reaction. The same ligation reaction can be split and used for ChIP analyses with different antibodies. 24. Add 4-10 µg of the antibody of choice
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
tapping. Rock for 5 min at 4°C. (24) Centrifuge 6600 rpm for 1 min at 4°C. Remove the buffer. (25) Add 1 ml of ice-cold High Salt Immune Complex Wash Buffer (EZ ChIP kit). Rock for 5 min at 4°C. (26) Centrifuge 6600 rpm for 1 min at 4°C
to be in the cross-linking step. MATERIALS Reagents Antibody ChIP cell lysis buffer ChIP dilution buffer ChIP hybridization buffer ChIP wash buffer Chloroform/isoamyl alcohol (24:1; prepare fresh before use
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