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- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit (24 reactions)
- 规格:
24次反应
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文献和实验Chromatin Immunoprecipitation (ChIP)
INTRODUCTION Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription
were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
Yeast Chromatin Immunoprecipitation (ChIP)
Yeast Chromatin Immunoprecipitation (ChIP)Hahn LabJuly 2003Chromatin IP MethodDay 11.Start 5 ml o/n cultures of strains to be testedDay 21.Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200
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