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- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
EpiQuik Chromatin Immunoprecipitation (ChIP) Kit (96 reactions)
- 规格:
96次反应
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文献和实验were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
Yeast Chromatin Immunoprecipitation (ChIP)
Yeast Chromatin Immunoprecipitation (ChIP)Hahn LabJuly 2003Chromatin IP MethodDay 11.Start 5 ml o/n cultures of strains to be testedDay 21.Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200
The ChIP (chromatin immunoprecipitation) method has emerged as a very powerful tool to study in vivo protein-DNA interactions
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