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- Candor
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- 德国
- 2025年12月03日
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- 英文名：
Assay Defender®
- CAS号：
N/A
- 保质期：
请询
- 供应商：
青木生物技术（武汉）有限公司
- 规格：
50 ml/125 ml/500 ml
| 规格： | 50 ml | 产品价格： | ￥1426.0 |
|---|---|---|---|
| 规格： | 125 ml | 产品价格： | ￥3174.0 |
| 规格： | 500 ml | 产品价格： | ￥11914.0 |
Assay Defender® 即用型分析缓冲液，用于减少因HAMA（人抗鼠抗体）、类风湿因子、非特异性结合、交叉反应、基质效应等造成的实验干扰。
Assay Defender® 可用于使用捕获抗体/包被抗体的免疫分析。尤其适用于含有HAMA（人抗鼠抗体）或其他AAA（抗动物抗体）的人或动物样本。
是一款即用型稀释缓冲液，适用于人或动物体液样本。使用简单，直接用Assay Defender®替代之前的稀释液即可。校正品也需用Assay Defender®稀释。样本稀释比 按照1：2或者更高。大多数应用中适宜的稀释比为1:10（1份样本+9份Assay Defender®） 。
Assay Defender® 可高效地防止干扰样本中的假阳性结果
使用ELISA方法，检测来自类风湿性疾病患者（样本1和2）的血清样本中肝癌细胞生物标记物hAxl的表达情况。样本3
使用ELISA方法，检测来自类风湿性疾病患者（样本1和2）的血清样本中肝癌细胞生物标记物hAxl的表达情况。样本3
是来自健康人的无干扰血清样本。样本分别用单独PBS-T/BSA、PBS-T/BSA +HAMA Blocker 1/HAMA Blocker 2 / HAMA
Blocker 3、CANDOR的Assay Defender®进行稀释。单 独 使 用 Assay Defender®即可完全消除干扰，因此对这两组干扰
样本给出正确的阴性结果 (< 25 ng/mL) 。 无 干 扰 的 样 本 结 果 不 受 Assay Defender®影响。
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文献和实验该产品被引用文献
- M. Roussel, Mikaël et al. (2021). Comparative immune profiling of acute respiratory distress syndrome patients with or wit- hout SARS-CoV2 infection. Cell Reports Medicine: 100291.
- D. M. Mrochen et al. (2021). Immune polarization potential of the S. aureus virulence factors SplB and GlpQ and modulation by adjuvants. Frontiers in immunology 12.
- M. Ebert et al. (2021). Identification of a modified coagulation factor X with enhanced activation properties as potential hemo- static agent. Blood Cells, Molecules, and Diseases 89: 102570.
- S. Zankovic et al. (2021). A Method for the Evaluation of Early Osseointegration of Implant Materials Ex Vivo: Human Bone Organ Model. Materials 14.11: 3001.
- A. Voskamp et al. (2021). Nasal systems immunology identifies inflammatory and tolerogenic myeloid cells that determine allergic outcome following challenge. medRxiv (2021): 2020-09.
- E. Torreggiani et al. (2021). Colorectal Carcinoma Affected Patients Are Significantly Poor Responders Against the Oncogenic JC Polyomavirus. Frontiers in Immunology 12: 1936.
- N. Reiners et al. (2021). Performance of a SARS CoV-2 antibody ELISA based on simultaneous measurement of antibodies against the viral nucleoprotein and receptor-binding domain. European Journal of Clinical Microbiology & Infectious Disea- ses, 1-5.
- H. Hayden et al. (2021). ELISA detection of MPO-DNA complexes in human plasma is error-prone and yields limited informati- on on neutrophil extracellular traps formed in vivo. PloS one, 16(4), e0250265.
- M. Becker, et al (2021). Immune response to SARS-CoV-2 variants of concern in vaccinated individuals. Nature Communica- tions, 12(1), 1-8.
- G. P. Anderson et al. (2021). Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid Protein. Anal. Chem. 2021, 93, 19, 7283–7291
- A. A. Urrutia et al. (2021). Inactivation of HIF-prolyl 4-hydroxylases 1, 2 and 3 in NG2-expressing cells induces HIF2-mediated neurovascular expansion independent of erythropoietin. Acta Physiologica, 231(1), e13547.
- C. Werner et al (2021). Exosomal Hsp70 in Liquid Biopsies-A Biomarker for Prediction and Response Monitoring in Cancer. doi: 10.20944/preprints202105.0524.v1
- K. Barkovits et al (2021). Protein Quantification Using the “Rapid Western Blot” Approach. Methods in Molecular Biology (Clif- ton, NJ) 2228 (2021): 29-39.
- M. Oggioni et al (2021). Long pentraxin PTX3 is upregulated systemically and centrally after experimental neurotrauma, but its depletion leaves unaltered sensorimotor deficits or histopathology. Scientific reports, 11(1), 1-17.
- D. Lobinger et al (2021). Potential Role of Hsp70 and Activated NK Cells for Prediction of Prognosis in Glioblastoma Patients. Frontiers in Molecular Biosciences, 8, 435.
- F. J. Hartmann et al. (2021). Single-cell metabolic profiling of human cytotoxic T cells. Nature biotechnology, 39(2), 186-197.
- S. Chevrier et al. (2021). A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Reports Medicine, 2(1), 100166.
- A. Shete et al (2021). A qualitative IgG ELISA for detection of SARS-CoV-2 specific antibodies in Syrian hamster serum samples. STAR protocols, 100573.
相关实验
样品和抗体稀释，这些亲和力鉴别溶液可以消除基于低或中等亲和力结合的所有干扰，同时保持特定信号。因此，它们大大减少了背景，提高了信噪比，并提高了结果的再现性（Fig.2）。与仅防止干扰性抗体干扰的HAMA阻断剂不同，LowCross Buffer®能够消除上述各种干扰。因此，这种基于亲和力鉴别的稀释剂可用于任何受到干扰的免疫分析。无需对分子基础进行耗时的更深入的研究，也无需仔细选择和滴定不同的HAMA阻断剂。在分析过程中，所有的结合反应都必须在亲和选择性条件下进行。由于许多诊断试剂盒需要长时间储存
*** Make sure the agar equilibrates to 55 C to 60 C before starting the assay; otherwise it will kill the cells. 3. Distribute several paper disks (Difco, #1599-33-6) into a sterile petri dish, and spot either 5 or 15 of synthetic alpha-factor (1 - 5 mg
Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
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Assay Defender®总HAMA/干扰阻断剂
￥1426 - 11914
