
BCA蛋白定量试剂盒
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- 技术资料
- 保存条件:
BCA蛋白定量试剂盒A、B应保存于2~8℃;5mg/mL BSA应保存于-20℃。有效期12个月。
- 保质期:
12个月
- 英文名:
-
- 库存:
现货
- 供应商:
爱必信(上海)生物科技有限公司
- CAS号:
-
- 规格:
2500T/500T
| 规格: | 2500T | 产品价格: | ¥975.0 |
|---|---|---|---|
| 规格: | 500T | 产品价格: | ¥325.0 |
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产品描述: 产品名称:BCA 蛋白定量试剂盒 描述: BCA法是理想的蛋白质定量方法,在碱性环境下蛋白质分子中肽键结构与Cu2+络合并将Cu2+还原成Cu1+。BCA特异地与Cu1+结合形成稳定的紫蓝色复合物,在562nm处有最大的光吸收值并与蛋白质浓度成正比,颜色的深浅与蛋白质的含量成正比,可以根据吸收值测定蛋白质浓度。该测定方法灵敏度高,操作简单,试剂及其形成的颜色复合物稳定性俱佳,并且受干扰物质去垢剂等影响小。 产品特点:
1、准确灵敏,线性范围广,BCA试剂的蛋白质测定范围是20~2000ug/mL;
2、快速:45min内完成测定,比经典的Lowry法快4倍而且更加方便;
3、不受样品中离子型和非离子型去污剂影响;
4、检测不同蛋白质分子的变异系数远小于考马氏亮蓝,可以酶标板法或分光光度计法测定蛋白质浓度。
组分:
储存/保存方法: BCA蛋白定量试剂盒A、B应保存于2~8℃;5mg/mL BSA应保存于-20℃。有效期12个月。 absin,您身边的科研百宝箱 |
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法是十分常用的蛋白质比色定量方法,很多试剂供应商都提供基于比色皿和微孔板的BCA蛋白定量试剂盒。BCA蛋白定量法相对于其它比色定量法具有操作简单、稳定、灵敏度高等优点。相对于蛋白质的固有的280nm吸收峰检测,BCA检测法提高了蛋白检测灵敏度和特异性,消除了核酸的干扰,核酸干扰在对细胞裂解物或其他生物样品进行蛋白定量时总是一个难以避免的干扰。在碱性条件下,蛋白将Cu2+ 还原为Cu+ ,Cu+ 与BCA 试剂形成紫色络合物,如图1,其吸光值与蛋白浓度成正比。测定在562nm 处的吸收值,并与标准
蛋白定量是生物学实验不可缺少的一部分。为验证细胞裂解是否成功,或为了将多个样品进行平行实验比较或标准化保存,需对细胞裂解液进行蛋白定量;为了判定蛋白的产量,需对纯化好的蛋白进行定量;为了将纯化好的蛋白用生物素或者报告酶进行标记,同样需首先对蛋白样品进行定量,以保证标记反应在适当的化学浓度下进行。现今有很多商品化的蛋白定量试剂盒,如BCA定量试剂盒等,操作起来,简便快捷。当然也有不少实验室,还是采用传统的方法进行蛋白定量。现将蛋白定量过程中所遇的一些问题小结一下。Q1: 采用考马斯亮蓝G5-20
对象为组织样品,取适量(250~500mg)新鲜组织样品或正确保存的组织样品,加1ml含蛋白酶抑制剂的总蛋白抽提试剂(或核蛋白抽提试剂),匀浆后抽提总蛋白(或核蛋白)。实验对象为细胞样品,每份样品取1×106~1×107细胞,PBS清洗细胞,去PBS加0.1ml~1ml含蛋白酶抑制剂的总蛋白抽提试剂(或核蛋白抽提试剂)抽提总蛋白(或核蛋白)。2. 蛋白质定量:按BCA蛋白质定量试剂盒操作说明操作,测定样品浓度。3. 变性聚丙烯酰胺不连续凝胶电泳(SDS-PAGE):将准备好的样品液和预染










