- ￥3000
- Worthington
- LS003741
- 美国
- 2026年01月20日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
Trypsin, TPCK Treated (TRTPCK)
- 规格：
500 mg
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- 内容
- 询问日期
文献和实验of interestSodium citrate (50 mM, pH 6.0)TPCK-modified trypsin (sequence-grade)EquipmentCompact reaction columns (CRC) and filters (35-µm pore size)Incubator (slow rotation, e.g., rotating wheel)MALDI mass spectrometer (e.g., the Voyager DE-STR) or equivalentThe
floating embryoid bodies. Cells are passaged as followed:- 1. All reagents are warmed to 37� Medium PBS PBS/EGTA TRYPSIN/EDTA 2. Remove medium 3. Wash with PBS (5ml/25cm2 flask, 10 ml/80cm2 flask), aspirate 4. Wash
Time Series Proteome Profiling
labeling by amino acids in cell culture (SILAC) is used in combination with crude ER fractionation, SDS–PAGE and LC-MS/MS to define altered protein expression in tunicamycin or thapsigargin treated cells versus untreated cells. Treated and untreated cells
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