- ￥1084
- worthington
- 美国
- LS003740
- 2025年07月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Store at 2-8°C. PROTECT FROM MOISTURE
- 保质期：
详见说明书
- 英文名：
Trypsin, TPCK Treated
- 库存：
大量现货供应
- 供应商：
上海经科化学科技有限公司
- CAS号：
TRTPCK
- 规格：
100 mg
Trypsin, TPCK Treated
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka and Carpenter, J. Biol. Chem. 239, 1799 (1964)].
名称：Trypsin, TPCK Treated
货号：LS003740
规格：100 mg
价格：1084
Code：TRTPCK
Activity/Specification：≥ 180 u/mg P (10,350 BAEE/3,450 USP/NF u/mgP)
UNSPSC Code(s)*：12352204
Source：Bovine Pancreas
Storage Conditions：Store at 2-8°C. PROTECT FROM MOISTURE.
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文献和实验of interestSodium citrate (50 mM, pH 6.0)TPCK-modified trypsin (sequence-grade)EquipmentCompact reaction columns (CRC) and filters (35-µm pore size)Incubator (slow rotation, e.g., rotating wheel)MALDI mass spectrometer (e.g., the Voyager DE-STR) or equivalentThe
floating embryoid bodies. Cells are passaged as followed:- 1. All reagents are warmed to 37� Medium PBS PBS/EGTA TRYPSIN/EDTA 2. Remove medium 3. Wash with PBS (5ml/25cm2 flask, 10 ml/80cm2 flask), aspirate 4. Wash
Time Series Proteome Profiling
labeling by amino acids in cell culture (SILAC) is used in combination with crude ER fractionation, SDS–PAGE and LC-MS/MS to define altered protein expression in tunicamycin or thapsigargin treated cells versus untreated cells. Treated and untreated cells
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