LB BROTH, MILLER,

Preparation of Broth and Plates, etc.

  Recipes:       1) LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O.  Swirl to dissolve, then add 110 µl of 10 N NaOH.  Autoclave.       2)   NZY Broth

Preparation of Broth and Plates

  Preparation of Broth and Plates, etc. [Use ddH2O 17 m* or better ] Recipes: 1) LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O. Swirl to dissolve, then add 110 µl of 10 N NaOH

LB培养基的配制

LB培养基，是微生物学实验中最常用的培养基，用于培养大肠杆菌等细菌，其分为液态或是加入琼脂制成的固态培养基。加入抗生素的 LB 培养基可用于筛选以大肠杆菌为宿主的克隆。 尽管该培养基的名称被广泛解释为Luria-Bertani 培养基，然而根据其发明人贝尔塔尼(Giuseppe Bertani)的说法，这个名字来源于英语的 lysogeny broth，即溶菌肉汤。LB培养