10X Tris-Glycine Transfer Buff

10X TBE BUFFER

  10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave

How to make DEPC-treated water and Tris Buffer

first, and then dissolve Tris to make the appropriate buffer. Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. However, its ability to form DNA:RNA or RNA:RNA

One-Dimensional SDS and Non-Denaturing Gel Electrophoresis of Proteins with Novex® Tris-Glycine Pre-Cast Gels

from the bottom of the cassette. 3)        In one smooth motion, gently pull the comb out of the cassette. 4)        Rinse the sample wells with the appropriate 1X SDS Running Buffer. Invert the gel and shake the gel to remove the buffer. Repeat two more times.