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大量
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北京诺博莱德科技有限公司
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伯乐Bio-Rad1610737 2x Laemmli Sample Buffer, 2x Laemmli样品缓冲液,产品简介:
产品品牌:伯乐Bio-Rad
产品名称:2x Laemmli样品缓冲液
英文名称:2x Laemmli Sample Buffer
产品货号:1610737
产品规格:30 ml
伯乐Bio-Rad1610737 2x Laemmli Sample Buffer, 2x Laemmli样品缓冲液,产品说明:
30 ml, premixed protein sample buffer for SDS-PAGE
30 ml,SDS-PAGE预混蛋白质样品缓冲液
使用2x Laemmli样品缓冲液制备SDS-PAGE样品。对于样品的还原,在与样品混合之前,向缓冲液中添加还原剂,例如2-shujiyichun。
2x Laemmli样品缓冲液可用于以下迷你蛋白和midi标准预制蛋白凝胶。
| Precast Protein Gel Type | Description |
| Mini-PROTEAN TGX Gels* | Laemmli-like, 12-month shelf life |
| Mini-PROTEAN TGX Stain-Free Gels* | Laemmli-like, image and analyze in less than 5 min, 12-month shelf life |
| Criterion TGX Gels* | Laemmli-like, 12-month shelf life |
| Criterion TGX Stain-Free Gels* | Laemmli-like, image and analyze in less than 5 min, 12-month shelf life |
| Criterion Tris-HCl Gels | Laemmli, 12-week shelf life |
| Criterion Stain Free Tris-HCl Gels | Laemmli, image and analyze in less than 5 min, 12-week shelf life |
| Criterion XT Tris-Acetate Gels* | For separation of large proteins, 8-month shelf life |
| Criterion Zymogram Gels | For protease analysis |
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伯乐Bio-Rad4x Laemmli Sample Buffer (1610747)4x Laemmli样品缓冲液(1610747)
伯乐Bio-Rad2-Mercaptoethanol (β-mercaptoethanol) (1610710)2-shujiyichun(β-shujiyichun)(1610710)
伯乐Bio-RadDithiothreitol (DTT), 1 g (1610610) and 5 g (1610611)二硫苏糖醇(DTT),1克(1610610)和5克(1610611)
伯乐Bio-RadTributylphosphine (TBP) (1632101)三丁基膦(TBP)(1632101)
伯乐Bio-Rad1610738 2x Native Sample Buffer, 30 ml
伯乐Bio-Rad1610732 10x Tris/Glycine/SDS, 1 L
伯乐Bio-Rad1610734 10x Tris/Glycine, 1L
如需其它容量的缓冲器请咨询本店客服
伯乐Bio-Rad1610737 2x Laemmli Sample Buffer,同类产品订购信息:
premixed Bffers for Tris HCI Gels
伯乐Bio-Rad Mini-PROTEAN Tris-Tricine Precast Gels
伯乐Bio-Rad Criterion Tris-Tricine Precast Gels迷你PROTEAN Tris Tricine预制凝胶
伯乐Bio-Rad Tris-Tricine预制凝胶标准
伯乐Bio-Rad 10x Tris/Tricine/SDS (1610744)10倍Tris/Tricine/SDS(1610744)
伯乐Bio-Rad Dithiothreitol (DTT) (1610610)二硫苏糖醇(DTT)(1610610)
伯乐Bio-Rad1610739Tricine Sample Buffer for Protein Gels, 30 ml同类产品订购信息:
伯乐Bio-Rad 1610739 2x Tricine Sample Buffer, 30 ml
伯乐Bio-Rad 1610744 10x Tris/Tricine/SDS, 1L
伯乐Bio-Rad 1610737 2x Laemmli Sample Buffer, 30 ml
伯乐Bio-Rad1610747 4x Laemmli Sample Buffer, 10 ml
伯乐Bio-Rad 1610738 2x Native Sample Buffer, 30 ml
伯乐Bio-Rad 1610732 10x Tris/Glycine/SDS, 1L
伯乐Bio-Rad 1610734 10x Tris/Glycine, 1L
伯乐Bio-Rad 1610772 10x Tris/Glycine/SDS. 5 L cube
伯乐Bio-Rad 1610771 10x Tris/Glycine, 5 L cube
Premixed Buffers for IEF Gels
伯乐Bio-Rad 1610763 IEF Sample Buffer, 30 ml
伯乐Bio-Rad 1610761 10x IEF Anode Buffer, 250 ml
伯乐Bio-Rad 1610762 10x IEF Cathode Buffer, 250 ml
Premixed Buffers for Zymogram Gels
伯乐Bio-Rad 1610764 Zymogram Sample Buffer, 30 ml
伯乐Bio-Rad 1610765 10x Zymogram Renaturation Buffer, 125 ml
伯乐Bio-Rad 1610766 10x Zymogram Development Buffer, 125 ml
Premixed Buffers for TBE and TBE-Urea Gels
伯乐Bio-Rad 1610767 5x Nucleic Acid Sample Loading Buffer, 10 ml
伯乐Bio-Rad 1610768 2x TBE-Urea Sample Buffer, 30 ml
伯乐Bio-Rad 1610770 10x Tris/Boric Acid/EDTA (TBE),5L cube
Accessories
1610992 Ready Gel Key Knife, free upon request with Ready Gel purchase
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文献和实验β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
to an equal volume of Assay 2X Buffer, which contains the substrate ONPG (o-nitrophenyl-beta-D-galactopyranoside). Samples are incubated for 30 minutes, during which time the Beta-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl
Preparation of protein extracts for western blot
with 2 ml 50 mM Tris,pH 7.5.,blot away excess liquid and freeze pellet on dry ice. 5.To frozen pellet add 0.2g 0.5 mm glass beads and 50 µl 2% SDS. 6.Vortex 90-120 sec full speed. 7.Place in boiling water bath for 3 min. 8.Add 100 µl 2x Laemmli sample
bath for 3 min. 8.Add 100 µl 2x Laemmli sample buffer(or use 75μl of 3X Laemmli sample buffer). 9.Place in boiling water bath for 1 min. 10.Spin briefly in Tomy (at room temp--15 sec,3K),remove liquid with a ml pippetteman tip,and transfer to 1.5 ml
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