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- 详细信息
- 文献和实验
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- 保质期:
2019/2/28
- 规格:
支
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
, Inc. ), the latter being less hazardous than the first. 1st injection: 15 - 50 ug Antigen in 200 ul adjuvant, injected subcutaneously. 2nd injection: 2 weeks later; boost with 15 - 50 ug of Antigen in adjuvant. (If time permits, boost again a month later). 7 - 10 days
Polyclonal and Monoclonal Antibodies
% ( 1000X ) Protocol: 1) ADSORBTION OF ANTIGEN a) Dilute Ag to 10 ug/ml in PBS. b) Add 100 ul of Ag solution to each well. c) Leave O.N covered with saran wrap, at 4 C. 2) BLOCKING a) Wash unbound Ag by inverting
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