应用举例 人原发卵巢肿瘤经gentleMACS Octo八通道组织解离器和Tumor Dissociation kit,human解离后,使用REAlease CD4/CD8 (TIL) MicroBeads, human 进行富集。将富集后的CD4+CD8肿瘤浸润T细胞采用如下REAfinity流式抗体组合进行免疫表型分析。
Specificity
Clone
Purpose
Fluorochrome
Detection filter
Product No.
CD3
REA613
T cell
FITC
525/50
130-113-138
CD4
REA623
CD4+ T cell
PerCP-Vio 770
725/40
130-113-228
CD8
REA734
CD8+ T cell
APC-Vio 770
785/62
130-110-681
CD39
REA739
Activation marker
PE-Vio 615
615/20
130-110-656
CD279(PD-1)
REA1165
Activation marker
PE
579/34
130-120-382
CD366 (TIM-3)
REA635
Activation marker
PE-Vio 770
785/62
130-121-334
CD137
REA765
Activation marker
APC
667/30
130-110-764
典型数据展示 Flow cytometric analysis of a human ovarian tumor sample. First, subsets of CD3+ T cells (A) were gated based on expression of CD4 and CD8 (B). The tumor-specific subpopulations CD279 (PD1)hi CD366 (TIM-)+ CD39+ CD8+ T cells and CD279 (PD1)+ CD39+ CD137+ CD4+ T cells were identified based on expression of different activation markers (PD-1, TIM-3, CD39, and CD137) within CD4+ and CD8+ T cells (C−F).