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- 英文名:
AMPINEXT™ DNA Library Preparation Kit (Illumina)
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50
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囊萤科技
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1*24Reactions
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
Summary: Illumina's Solexa Sequencing Technology
fragments of the size range of 150-200bp are isolated via gel extraction and amplified using limited cycles of PCR.Complete detailed protocols for DNA and small RNA library preparation can be found in the documents provided in the attachments to this post
Multiplex Illumina Sequencing Using DNA Barcoding
Figure 7.11.1 Outline for preparation of 5′ barcoded DNA libraries for Illumina sequencing. DNA fragments ranging from 100 bp to 500 bp are first subjected to end‐repair, 5′ phosphorylation, and 3′ A base addition. Y‐adapters with unique barcode
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