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QIAGEN
提供免疫学,细胞学,分子生物学,微生物学,即用型试剂,常规生化试剂一站式供应平台及IHC,IF,WB,ELISA方面技术支持。品牌包括:ATCC,CMCC,abcam,BD,R&D,CST,Ebioscience,Invitrogen(gibco、MP,Dynal,Biosource,caltag,zymed、icfc),Fermentas(MBI),Merck-Millipore,Peprotech,Pierce,Promega,Sigma ,Qiagen,Roche,Selleckchem,Amresco,Hyclone,Santa Cruz,Abgent
上海北诺生物科技有限公司成立于2007年,是由留美生物医学界专家与国内生命科学界知名专家共同创办,由中科院健康科学研究所、上海市免疫学研究所、上海市内分泌研究所、上海市血液学研究所、上海交通大学、上海交通大学医学院附属瑞金医院等生命科学研究专家提供技术支持和理论指导。
北诺生物精选国际一流品牌和国内经过认证的、质量较为可靠、有质量体系管理的产品供应商的产品,能够根据课题需要,提供基于免疫学、分子生物学和细胞生物学,微生物学产品组合。通过进口产品和国内精选、质量可靠的产品组合,保证您的课题能顺利、甚至高质量完成。
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| PVDF Western Blotting Membrane | Roche | 3010040001 | 1 roll (30 cm x 3.00 m) |
| Proteinase K,recomb.,PCR Grd.S | Roche | 3115887001 | 1.25 ml |
| Genopure Plasmid Midi Kit | Roche | 3143414001 | RT*1 kit (for up to 20 preparations) |
| Genopure Plasmid Maxi Kit | Roche | 3143422001 | RT*1 kit (for up to 10 preparations) |
| Terminal Transferase, recomb. | Roche | 3333574001 | 24,000 U (for 60 tailing or 3'-end labeling reactions) |
| Protector RNase Inhibitor, 10 | Roche | 3335402001 | 10,000 U (5 x 2,000 U) FR |
| DIG-11-UTP,LSg.,3,5mM,200nmol | Roche | 3359247910 | 200 nmol (3.5 mM, 57 ?l) |
| Proteinase K recombinant PCR Grade | Roche | 3508838103 | 100mg (2°to 8°) |
| cOmplete,EASYpack,20 Tab. | Roche | 4693116001 | 20 tablets (in foil blisters) |
| cOmplete,EDTA-free,EASYpack,20 | Roche | 4693132001 | 20 tablets (in foil blisters) |
| cOmplete,Mini,EDTA-free,EASYpa | Roche | 4693159001 | 30 tablets (in foil blisters) |
| LightCycler 480 SYBR Green I Master, 5 x 1 ml | Roche | 4707516001 | 5×100反应(20 μl) FR |
| FastStart PCR Master 10 ml | Roche | 4710444001 | 8 x 1.25 ml FR |
| LightCycler 480 Multiwell Plate 96 | Roche | 4729692001 | 5 x 10 块板 (含封板膜) |
| LightCycler 480 Multiwell Plate 384 | Roche | 4729749001 | 5 x 10 块板 (含封板膜) |
| Cytotoxicity Det.Kit PLUS (LDH | Roche | 4744926001 | 1 kit (400 tests in 96 wells)*FR |
| LightCycler 480 SYBR Green I Master, 10 x 5 ml | Roche | 4887352001 | 10×500反应(20 μl)*FR |
| Transcriptor cDNA Synth. Kit 1 | Roche | 4896866001 | 1 kit (100 reactions)*FR |
| Transcriptor cDNA Synth. Kit 2 | Roche | 4897030001 | 1 kit (200 reactions)*FR |
| PhosStop, 20 Tablets | Roche | 4906837001 | 20 tablets (each tablet for 10 ml) |
| PhosStop, 10 Tablets | Roche | 4906845001 | 10 tablets (each tablet for 10 ml) |
| FS Universal SYBR Green Master | Roche | 4913850001 | 4 x 1.25 ml (for 200 reactions of 50 ?l final reaction volume) |
| FastStart Universal Probe Mast | Roche | 4913949001 | 2 x 1.25 ml (for 100 reactions of 50 ?l final reaction volume) |
| FastStart Universal Probe Mast | Roche | 4914139001 | EA |
| LightCycler Capillaries (20 ?l) | Roche | 4929292001 | 5x 96根/盒 |
| Dispase II (neutral protease, | Roche | 4942078001 | 5 x 1 g CR |
| Cell Proliferation Reagent WST | Roche | 5015944001 | 8 ml |
| LightCycler 480 Multiwell Plate 384, clear | Roche | 5102430001 | 5 x 10 块板 (含封板膜) |
| E-Plate 16 (6x6 plates) | Roche | 5469813001 | 6x6 plates RT |
| E-Plate 16 (6 plates) | Roche | 5469830001 | 6 plates |
| cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack | Roche | 5892791001 | 30 tablets |
| cOmplete ULTRA Tablets, Mini, EASYpack | Roche | 5892970001 | 30 tablets |
| X-Tremegene Hp Dna Transf.Reag.Trial | Roche | 6365752001 | trial pack |
| X-tremeGENE 9 DNA Transf. Reag. 1.0 ml | Roche | 6365787001 | 1.0 ml*CR |
| X-tremeGENE HP DNA Transf. Reag. 1.0 ml | Roche | 6366236001 | 1.0 ml |
| DNase I, Grade II | Roche | 10104159001 | 100 mg |
| Pronase from Streptomyces gris | Roche | 10165921001 | 1 g (non-sterile) CR |
| DAPI | Roche | 10236276001 | 10 mg RT |
| Polyethylene Glycol 1500 (PEG | Roche | 10783641001 | 10 x 4 ml |
| Endoproteinase Lys-C Seq. Grad | Roche | 11047825001 | 3 x 5 ?g CR |
| Anti-digoxigenin AP-conjungate | Roche | 11093274910 | 150 U (200 ?l) |
| Dig-RNA labeling kit 1St | Roche | 11175025910 | 1 kit (2 x 10 labeling reactions) |
| Anti-digoxigenin-fluorescein | Roche | 11207741910 | 200 ?g |
| DIG-11-UTP | Roche | 11209256910 | 250 nmol (10?mM, 25 ?l) |
| Protein G-Agarose | Roche | 11243233001 | 5 ml |
| DIG RNA Labeling Mixture, 10x | Roche | 11277073910 | 40 ?l (20 reactions) |
| Mycoplasma Detection Kit | Roche | 11296744001 | 1 kit (25 tests) |
| Nylon Membranes, 1 roll | Roche | 11417240001 | 1 roll (0.3 x 3 m) |
| PCR DIG labeling Mix 1pc | Roche | 11585550910 | 500 ?l (2 x 250 ?l) (for 2 x 25?reactions of 100 ?l final reaction volume) |
| DIG High Prime Lab./Det.Kit II | Roche | 11585614910 | 1 kit (12 labeling reactions and 24 detection reactions) |
| DIG Wash and Block Buffer Set | Roche | 11585762001 | 1 set (30 blots) |
| DIG Easy-Hyb | Roche | 11603558001 | 500 ml |
| PCR Dig Probe Synthesis 1pc | Roche | 11636090910 | 1 kit (25 reactions) |
| Cell Proliferation Reagent WST | Roche | 11644807001 | 25 ml (2,500 tests) |
| Cell Proliferation ELISA,BrdU | Roche | 11647229001 | 1 kit (1,000 tests) |
| TRIPURE ISOLATION REAGENT 200 | Roche | 11667165001 | 200 ml |
| NBT/BCIP Stock Solution | Roche | 11681451001 | 8 ml |
| In Situ Cell Death fluorescein | Roche | 11684795910 | 1 kit (50 tests) |
| In Situ Cell Death POD | Roche | 11684817910 | 1 kit (50 tests) |
| Fluorescein RNA Label.Mix 10x | Roche | 11685619910 | 40 ?l (20 reactions) |
| Expand HiFi PCR Sys., 100 u | Roche | 11732641001 | 100 U |
| Expand HiFi PCR Sys., 500 u | Roche | 11732650001 | 500 U (2 x 250 U) |
| DIG High Prime Lab/Detection K | Roche | 11745832910 | 1 kit (12 labeling reactions and 24 detection reactions) |
| HP Plasmid Isolation Kit, 50 p | Roche | 11754777001 | 1 kit (50 purifications) |
| Cell Death Detection ELISA^PLU | Roche | 11774425001 | 1 kit (96 tests) |
| HP PCR Template Preparation Ki | Roche | 11796828001 | 1 kit (100 purifications) |
| MINI QUICK SPIN RNA COLUMNS | Roche | 11814427001 | 50 columns |
| Anti-GFP | Roche | 11814460001 | 200 ?g |
| HP RNA Isolation Kit | Roche | 11828665001 | 1 kit (50 reactions) |
| b-Gal Staining Set | Roche | 11828673001 | 1 set (for 100 tests in 3.5-cm dishes) |
| Annexin V FLUOS Staining Kit | Roche | 11858777001 | 1 kit (50 tests) |
| Anti-HA High Affinity (3F10), | Roche | 11867423001 | 50 ?g *CR |
| CDP-Star, ready-to-use | Roche | 12041677001 | 2 x 50 ml |
| In Situ Cell Death TMR | Roche | 12156792910 | 1 kit (50 tests) |
| Anti-HA-Biotin, High Affinity | Roche | 12158167001 | 50 ?g CR |
| Restr.-Endonucl. Sma I, 1000 U | Roche | 10220566001 | 1,000 U (10 U/Ul) |
| Restr.-Endonucl. Bam HI, 1000u | Roche | 10220612001 | 1,000 U (10 U/Ul) |
| Aprotinin, 10mg | Roche | 10236624001 | 10 mg |
| DNA Ligase, T4, 100units | Roche | 10481220001 | 100 U (1 U/Ul) |
| Restr.-Endonucl. Pst I, 3000 U | Roche | 10621625001 | 3,000 U (10 U/Ul) |
| Restr.-Endonucl. Hind III, 500U | Roche | 10656313001 | 5,000 U (10 U/μl) |
| Restr.-Endonucl. Eco RV, 2000 | Roche | 10667145001 | 2,000 U (10 U/Ul) |
| Restr.-Endonucl. Xba I, 1000 U | Roche | 10674257001 | 1,000 U (10 U/μl) |
| Restr.-Endonucl. Xho I, 5000 U | Roche | 10899194001 | 5,000 U (10 U/μl) |
| Anti-Digoxigenin-AP, Fab fragments | Roche | 11093274910 | 150 U (200ul) |
| Blocking Reagent 50 g | Roche | 11096176001 | 50 g |
| Restr.-Endonucl. Eco RI | Roche | 11175084001 | 10,000 U (10 U/Ul) |
| DIG-11-UTP | Roche | 11209256910 | |
| Collagenase P | ROCHE | 11213857001 | 100mg |
| DIG RNA Labeling Mixture, 10x 40 ?l (20 reactions) | Roche | 11277073910 | |
| Mycoplasma Detection Kit | ROCHE | 11296744001 | 1 kit (25 tests) |
| BM Chem-Lum. WB-Substr. (POD) | Roche | 11500694001 | 1 set (4,000 cm2 memb |
| BM Chem-Lum. WB-Substr. (POD) | Roche | 11500708001 | 1 set (1,000 cm2 memb |
| DIG Wash and Block Buffer Set 1 set (30 blots) | Roche | 11585762001 | 1 set (30 blots) |
| DIG Easy-Hyb 500 ml | Roche | 11603558001 | |
| Rapid DNA Ligation Kit 1 kit | Roche | 11635379001 | 40 DNA ligation reacti |
| Cytotoxicity Detection Kit (LD 1 kit | Roche | 11644793001 | (2,000 tests) |
| In Situ Cell Death fluorescein 1 kit (50 tests) | Roche | 11684795910 | 1 kit (50 tests) |
| In Situ Cell Death POD 1 kit (50 tests) 5899 | Roche | 11684817910 | 1 kit (50 tests) |
| Fluorescein RNA Label.Mix 10x | Roche | 11685619910 | 40 Ul (20 reactions) |
| Streptavidin, recombinant, 1 m | Roche | 11721666001 | 1 mg |
| Expand HiFi PCR Sys., 100 u | Roche | 11732641001 | |
| Expand HiFi PCR Sys., 500 u | Roche | 11732650001 | 500 U (2 x 250 U) |
| HP PCR Template Preparation Kit | ROCHE | 11796828001 | 1 kit (100 purifications) |
| Anti-GFP | Roche | 11814460001 | 200 Ug |
| HP RNA Isolation Kit | Roche | 11828665001 | 1 kit (50 reactions) |
| Titan One Tube RT-PCR System(1 | Roche | 11855476001 | 100 reactions |
| HP Viral Nucleic Acid Kit | Roche | 11858874001 | 1 kit (for up to 100 purif |
| FuGENE 6 Transf. Reag., 1 ml | Roche | 11988387001-1ml | ##1ml(300 transfections) |
| FuGENE 6 Transf. Reag., 1 ml | Roche | 11988387001-1ml | 1ml(300 transfections) |
| Lumi-Light^PLUS WB Substrate | Roche | 12015196001 | 100 ml (1,000 cm2 membr |
| CDP-Star, ready-to-use 2 x 50 ml | Roche | 12041677001 | 2 x 50 ml |
| MagNA Pure LC Tip Stand | Roche | 3004155001 | 200 个 |
| Genopure Plasmid Maxi Kit | Roche | 3143422001 | 1 kit (for up to 10 prepara |
| Protector RNase Inhibitor, 10 | Roche | 3335402001-1ml | 2000 U*FR |
| Pwo SuperYield DNA Polymerase | Roche | 4340868001 | 100 U |
| siRNA Transfection Reagent | Roche | 4476115001-1ML | 1ML |
| FastStart TaqMan Probe Master | Roche | 4673409001 | 2 x 1.25 ml (for 100 r |
| cOmplete,Mini,EASYpack,30 Tab. | Roche | 4693124001 | 30 tablets (in foil blisters) |
| cOmplete,EDTA-free,EASYpack,20table | Roche | 4693132001 | |
| FuGENE HD Transfection Reagent | Roche | 4709713001-1ml | ##1ml |
| FastStart PCR Master 10 ml | Roche | 4710444001 | 8 x 1.25 ml (for 400 react |
| DNase I recombinant, RNase-fre | Roche | 4716728001 | 10,000 U |
| Complete Lysis, mammalian | Roche | 4719956001 | 1 kit (200 ml lysis reagent |
| Complete Lysis, mammalian EDTA | Roche | 4719964001 | 1 kit 200 ml lysis re |
| LightCycler 480 Multiwell Plate 96 | Roche | 4729692001 | 5 x 10 块板 |
| LightCycler 480 Multiwell Plate 96 | Roche | 4729692001-1pk | 1pk |
| LightCycler 480 Multiwell Plate 384 | Roche | 4729749001 | 5 x 10 块板 |
| LightCycler? 480 Multiwell Plate 384 white | Roche | 4729749001-1plate | ##1 plateRT |
| LightCycler 480 Sealing Foil | Roche | 4729757001-1pk | 1片 |
| FastStart Taq DNA Pol. dNTPack | Roche | 4738420001 | 5,000 U (20 x 250 U) |
| Expand HiFi PLUS PCR System dN | Roche | 4743733001 | 500 U (2 x 250 U) |
| PhosStop, 10 Tablets | Roche | 4906845001 | 10 tablets (each tablet for 10 m |
| FS Universal SYBR Green Master(Rox) | Roche | 4913914001-5ml | ##5ml/pk |
| FS Universal SYBR Green Master | Roche | 4913914001-5ML | 5 ml (for 2,000 r |
| Dispase II (neutral protease | Roche | 4942078001 | 5 x 1 g |
| Transcriptor HiFi cDNA Synth. | Roche | 5081955001 | 1 kit (50 reactions, i |
| Transcriptor HiFi cDNA Synth. | Roche | 5091284001 | 1 kit (100 reactions) |
| LightCycler 480 Multiwell Plate 384, clear | Roche | 5102430001-1pk | 1plate |
| cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack | Roche | 5892791001 | 30 tablets |
| cOmplete ULTRA Tablets, Mini, EDTA-free, EA | Roche | 5892791001-10tablets | 10tablets |
| cOmplete ULTRA Tablets, Mini, EASYpack | ROCHE | 5892970001 | 30 tablets |
| X-tremeGENE 9 DNA Transf. Reag. | Roche | 6365809001-1ml | ##1.0 ml CR |
| X-tremeGENE 9 DNA Transf. Reag. | Roche | 6365809001-1ML | 1.0 ml |
| X-tremeGENE HP DNA Transf. Reag. | Roche | 6366546001-1ml | ##1.0 ml*FR |
| QIAprep Spin Miniprep Kit (50) | Qiagen | 27104 | ea |
| QIAquick PCR Purification Kit (50) 50T | Qiagen | 28104 | ea |
| Ni-NTA Agarose (25 ml) | Qiagen | 30210 | ea |
| Ni-NTA Superflow (25 ml) | Qiagen | 30410 | ea |
| QIAamp DNA Blood Mini Kit (50) | Qiagen | 51104 | ea |
| QIAamp DNA Blood Midi Kit (100) 100T | Qiagen | 51185 | ea |
| EpiTect Bisulfite Kit (48) | Qiagen | 59104 | ea |
| RNeasy Mini Kit (50)50T | Qiagen | 74104 | ea |
| RNeasy Plant Mini Kit (50) | Qiagen | 74904 | ea |
| QuantiFast SYBR Green PCR Kit (80) | Qiagen | 204052 | ea |
| QuantiFast SYBR Green PCR Kit (400) | Qiagen | 204054 | ea |
| miRNeasy Mini Kit (50) | Qiagen | 217004 | ea |
| miScript SYBR Green PCR Kit (200) | Qiagen | 218073 | ea |
| miScript II RT Kit (50) | Qiagen | 218161 | ea |
| miScript PCR Starter Kit (80) | Qiagen | 218193 | ea |
| Attractene Transfection Reagent (1 ml) | Qiagen | 301005 | ea |
| Attractene Transfection Reagent (4 x 1 ml) | Qiagen | 301007 | ea |
| HiPerFect Transfection Reagent 0.5ml | Qiagen | 301704 | ea |
| HiPerFect Transfection Reagent (1 ml) | Qiagen | 301705 | ea |
| HiPerFect Transfection Reagent (4 x 1 ml) | Qiagen | 301707 | ea |
| Matrix Standard BT5 multi cap. (50) | Qiagen | 386125 | ea |
| QX DNA Size Marker 100bp - 2.5kb (50 ?l) | Qiagen | 929559 | ea |
| Filter-Tips, 200 ul (1024) | Qiagen | 990332 | ea |
| Disposable Filter-Tips, 1000 ul (1024) | Qiagen | 990352 | ea |
| Rotor Adapters (10 x 24) | Qiagen | 990394 | ea |
| Attractene Transfection Reagent (0.1 ml) each | Qiagen | 1051561 | ea |
| RBC Lysis Solution (450 ml) | Qiagen | 158902 | ea |
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文献和实验【共享】经验:The RNeasy Mini Kit from Qiagen
The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic format is similar to other Qiagen products designed to purify nucleic acids: ion exchange columns specifically bind RNA which is purified by wash steps
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
.) to 70 µl Buffer RDD. Mix gently. 8. Add the DNase I incubation mix (80 µl) directly onto the RNeasy membrane, 20–30°C for 15 min. 9. Run 700µl RW1 through the mini column, 15 seconds at 12,000 rpm. 10.
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
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