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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
- 英文名:
Recombinant Escherichia coli (strain K12) Crossover junction endodeoxyribonuclease RuvC
- 库存:
1
- 供应商:
北京博蕾德国际贸易有限公司
- 规格:
10ug//20ug//50ug//100ug
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文献和实验strategies for strain design. We focus on the use of the polymerase chain reaction (PCR) for gene amplification, plasmid DNA for recombinant gene cloning and expression, and homologous recombination and phage transduction for modifying chromosomal DNA
/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time
Expression of Recombinant Proteins with Uniform N-Termini
Heterologously expressed proteins in Escherichia coli may undergo unwanted N-terminal processing by methionine and proline aminopeptidases. To overcome this problem, we present a system where the gene of interest is cloned as a fusion
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