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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
欣博盛生物科技有限公司
- 检测范围:
1.95-125pg/ml
- 检测方法:
双抗体检测方法
- 应用:
ELISA试剂盒
- 适应物种:
人
- 标记物:
HRP标记
- 样本:
血清/ 血浆/ 细胞培养上清
- 规格:
96T*10
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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