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- WBKLS0500
- 2025年07月16日
- Western Blotting
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Luminata™ Western HRP SubstratesPremixed for convenience.Formulated for optimal results. The new family of Luminata HRP chemiluminescent substrates is premixed for convenience. Just pour directly onto the Western membrane without worrying about pipetting error. Luminata substrates cover a range of sensitivities for all your needs. |
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Millipore’s Reagents for Western BlottingFor simple, cost-effective protein detection, use Millipore's Western blotting reagents to achieve the highest sensitivity with the least background. |
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Overview of the SNAP i.d.® Protein Detection SystemSuperior Blots in a SNAP! |
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详细描述见链接：http://www.millipore.com/catalogue/item/WBKLS0500
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文献和实验Lumigen HyPerBlu provides sensitive chemiluminescent detection of hydrogen peroxide
Abstract Lumigen HyPerBlu reagent is a one solution chemical substrate based on dioxetane chemiluminescent technology. With HyPerBlu reagent, a direct reaction with hydrogen peroxide produces an intense sustained luminescent signal making
,000) prior to visualization with Immobilon Western HRP Chemiluminescent Substrate. The blots were then stripped by (B) low pH or (C) heat/detergent and re-exposed to the secondary antibody and the Immobilon Western substrate to demonsrate complete
液裂解细胞，用移液器适当吹打使细胞脱落，大瓶贴壁细胞可以先用胰酶洗脱下来后再裂解，裂解方法仿照悬浮细胞处理。 B、 悬浮细胞的处理：先将悬浮细胞置于 EP 管中 3000 rpm 离心 5 min 去掉上清培养基，每 20 μL 体系细胞加 100 μL 裂解液反复吹打至细胞完全破碎。 处理好的样品 12000 rpm 离心 5 min，取上清即为提取的蛋白。置于 4 ℃ 可保存一周，-20℃ 可保存约 2 个月，-80 ℃ 可保存半年。 蛋白浓度的测定：酶标板，酶标仪、离心机、1 mg/mL
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