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RiOs 30/50/100/150/200 Water Purification SystemsReverse osmosis water purification systemsRiOs reverse osmosis systems are designed to produce up to 8000 L/day of Type III laboratory-grade water from potable tap feedwater. |
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S.D.S. Systems – Purified Water Storage & DistributionStore 200 to 350 Liters of pure waterMillipore’s compact S.D.S. Storage and Distribution Systems are designed to store 200 to 350 liters of purified water produced by Elix or RiOs systems, and to maintain consistent purity of stored water, provide effective protection against airborne contamination and distribute the purified water under pressure to multiple-use locations. |
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Elix 20/35/70/100 Water Purification SystemsType 2 analytical-grade waterElix systems are designed to produce up to 4000 L/day of Type 2 analytical-grade water from potable tap feedwater to meet or exceed the relevant standards defined by CLSI, ISO® 3696 / BS 3997 and the European and US Pharmacopoeia. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/TANK60350
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文献和实验摇晃几下,润一遍,弃掉这些胶液,这时候尽量去干净,动作要快。再正式灌满胶液,插上梳齿。 跑电泳时,可以60V先压约30min,可以看到溴酚蓝变的又细又直,待进入分离胶,可以看到预染的Marker已经分离,就可以将电压调至120V跑完。 转膜与普通的都一样,这里建议30V恒压转2小时(用于80KD以下的蛋白分子),如果有更大的蛋白280KD的mTor,可以再这2h后再加350mA 30min,效果很好,280KD以下90KD以上的都可以加350mA这一步,时间
【原创】Tricine SDS-PAGE凝胶配方(16.5%)
吧^_^)5.100%甘油6.样品缓冲液1ml甘油(100%),0.5ml巯基乙醇,1ml 0.5mol/L Tris-HCL(PH6.8,6.05gTris-HCL溶于100ml水中),溴芬兰痕量(0.005%~0.02%),加水至5ml水中,分装成1ml大小,-20摄氏度保存。二、Tricine SDS-PAGE凝胶配方(16.5%)30ml体系(小板胶10ml到15ml即可,spacer为0.5mm-0.75mm):丙稀酰胺母液 (49.5%T,6%C) 10ml凝胶缓冲液液(3X) 10ml100%甘油
【我们实验室的SDS-PAGE改良集锦】蛋白SDS-PAGE电泳及定量在《蛋白质技术手册》基础上,根据实验条件的方便修改。请仔细看各种细节改良,各种操作效果经本实验室5年验证。【一】 储存液配制(均4℃冰箱保存,用完放回)(1)2 M Tris-HCl (实测pH 8.9±0.1,25℃) 500 mL:121 g Tris base加350 mL双蒸水溶解,以玻璃棒搅拌约1 min,缓慢加入浓盐酸(11.8 M)20 mL,继续搅拌均匀,并使Tris全部溶解,溶液澄清,加入适量双蒸水至500
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