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Millipore 冷冻小鼠皮质神经干细胞 干细胞培养 小鼠

神经干细胞
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  • SCR029
  • 2025年07月10日
  • Stem Cell Culture
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    • 文献和实验
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    • 应用范围

      Stem Cell Culture

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      1 vial

    Description:
    Cryopreserved Mouse Cortical Neural Stem Cells
    Trade Name:
    Chemicon (Millipore)
    Product Overview:
    Millipore's Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.

    We recommend that Millipore's Cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population.

    For Research Use Only; not for use in diagnostic procedure.
    Key Applications:
    Stem Cell Culture
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Species:
    Mouse
    Stem Cell Type:
    Neural Stem Cells
    Stem Cell Workflow Stage:
    Cells/Isolation
    Cell Line Type:
    Neural Stem Cells
    Components:
    1x10e6 viable Mouse Cortical Neural Stem Cells: (Part No. 2004115) derived from mixed gender C57/BL6 embryonic day 15-18 mice, cryopreserved. Store in liquid nitrogen.
    Storage Conditions:
    When stored at the recommended storage conditions (liquid nitrogen), mouse cortical neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
    Packaging:
    1 x 106 cells
    Materials Required but Not Delivered:
    1. Neural Stem Cell Expansion Medium (Millipore Catalog No. SCM003)

    2. Basic fibroblast growth factor (bFGF, FGF-2; Specific Activity

    > 2 x 10e6 Units/mg, Millipore Catalog No. GF003)

    3. Epidermal Growth Factor (EGF; Specific Activity > 1 x 10e7 Units/mg; Millipore Catalog No. GF001)

    4. Heparin (Sigma Catalog No. H-1027)

    5. Poly-L-ornithine (Sigma Catalog No. P3655)

    6. Laminin (Sigma Catalog No. L-2020)

    7. ESGRO Complete™ Accutase™ (Millipore Catalog No. SF006)

    8. Tissue culture-ware

    9. Phosphate-Buffered Saline (1X PBS) (Millipore Catalog No. BSS-1005-B)

    10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)

    11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)

    12. Primary and secondary antibodies

    13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution

    14. Anti-fading mounting solution (DABCO/PVA)

    15. Hemacytometer

    16. Microscope

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/SCR029

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    图标文献和实验
    相关实验
    • 胚胎小鼠纹状体神经干细胞的分离培养及鉴定

      -17AC CO2 培养箱 SANYOXTD-10A体视显微镜 芜湖光学仪器厂无菌超净工作台 苏州净化设备厂50ml 细胞培养瓶 NUNC96孔细胞培养板 NUNC24孔细胞培养板 NUNC6 孔细胞培养板 NUNC倒置显微镜 LeicaOLYMPUS荧光显微镜 OLYMPUSOLYMPUS光学显微镜 OLYMPUS方法: 神经干细胞的培养 ①原代细胞的培养:取孕13.5天昆明种二级孕小鼠,引臼脱颈处死,碘酒、乙醇消毒腹部,逐层剖开腹部皮肤、肌肉、腹膜,取出子宫,放入盛有D1

    • 神经干细胞的培养

      一、 神经干细胞的分离和传代 无菌条件下取新生SD大鼠(出生48h内)脑组织,D-Hanks液充分漂洗后,在解剖显微镜下剥离脑膜,准确分离海马,用眼科剪将海马剪碎后,再转移到DMEM/F12(1:1)加B27 和bFGF(20ng/ml)的无血清培养基,吸管吹打机械分离制作单细胞悬液,台盼蓝染色后细胞计数,调整细胞浓度为5×104~5个/ml,置于24孔培养板中培养,每孔加入细胞悬液500μl。待神经球形成后再次机械分离克隆制作单细胞悬液,仍以5×104个/ml的细胞浓度置于24

    • 神经干细胞

      神经干细胞是一种终身具有自我更新能力的细胞,其子细胞能分化产生神经系统的各类细胞,干细胞经过不对称分裂产生一个祖细胞和另一个干细胞,祖细胞具有有限的自我更新能力,并自发分化产生成神经元细胞和成胶质细胞等,从而生成神经元及神经胶质细胞。     以往认为中枢神经系统的神经元再生于出生前或出生后不久就停止了。近年的一些研究表明成年哺乳动物的脑组织仍然可不断产生新的神经元,并且证实在人脑组织中同样存在神经干细胞。因此,人们对神经系统再生的机理

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