- 询价
- SCM007
- 2025年07月16日
- Stem Cell Culture
- 0
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Stem Cell Culture
- 宿主：
0
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
0
- 规格：
50 mL
- Cell Culture Supplements
- Stem Cell Reagents
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/SCM007
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文献和实验Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells
pellet (gently) in 3 ml ES media to wash away collagenase. Spin 5 minutes at 1000 rpm. Resuspend pellet (again, gently!) in 0.25 ml Resuspension Medium per vial (This is one half the final volume required for freezing). Dropwise, add
ES and TS cell freezing/thawing
a multichannel pipettor change the medium 2-3 hours prior to freezing. 2. Freshly prepare 2x cell freezing media. 3. Aspirate the medium from each well and wash the cells with PBS (approximately 200 ml). 4. Add 50 microliter trypsin
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Trypsin/EDTA (0.5%) Equipment Centrifuge Centrifuge tubes (50-mL) Equipment for harvesting bone marrow from mouse femurs Filter membranes (0.45-µm) Flow cytometry system capable of cell sorting and three-color
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