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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海博湖
- 检测范围:
78-5000pg/ml
- 检测方法:
Sandwich ELISA, Double Antibody
- 应用:
46.9pg/ml
- 样本:
AOPEP
- 规格:
96T
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文献和实验PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
with patterns that match those of rodent T1α. However, the human data are unclear, because antibodies to rodent T1α do not recognize ATI cell antigens in normal adult human lung. This likely indicates that O-glycosylation of the human protein(s
【分享】Human press METHODS IN MOLECULAR BIOLOGY
, Bret A., 1995 41.Signal Transduction Protocols, 1st. Edited by D. A. Kendall, 1995 42. ELISA Theory and Practice. Edited by Crowther, John R., 1995 43. In Vitro Toxicity Testing Protocols, Edited by O'Hare, Sheila, 1995 44. Agrobacterium
。在ELISA中,DH2一般为无色化合物,经酶作用后成为有色的产物,以便作比色测定。常用的供氢体有邻苯二胺(O-phenylenediamine,OPD)、四甲基联苯胺(3,3',5,5'-tetramethylbenzidine,TMB)和ABTS[2,2'-azino-di-(3-ethylbenziazobine sulfonate-6)]。 OPD氧化后的产物呈橙红色,用酸终止酶反应后,在492nm处有最高吸收峰,灵敏度高,比色方便,是HRP结合物最常用的底物。OPD本身难溶于水,OPD•2HCL
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