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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海博湖
- 检测范围:
0.78-50ng/ml
- 检测方法:
Sandwich ELISA, Double Antibody
- 应用:
0.469ng/ml
- 样本:
BCO2
- 规格:
96T
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文献和实验1 to a tube. 2) Add 20 μl of Antibody Mix. 3) Mix well and incubate for 20 min at 2–8°C. 4) Wash the cells by adding 2 ml Buffer 1. Mix well by tilting the tube several times and centrifuge at 300 x g for 10 min at 2–
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
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