K108661P Anti-KEAP1 Polyclonal

【抗体靶点简介】

Substrate-specific adapter of a BCR E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation. The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome.

【实验操作视频参考】

Western blot实验参考操作流程

Immunohistochemistry实验参考操作流程

抗体在科研领域应用十分广泛，对解析生物学过程、探究疾病发病机制、创新药物研发与临床治疗研究均起到关键支撑作用。

索莱宝现有30000余种经过严格实验验证的抗体产品，全面覆盖神经科学、细胞凋亡、表观遗传学等热门研究方向，适配 WB、IHC、ELISA、IP、co-IP 等主流实验技术场景。包括内参标签抗体，以及多种标记类型的一抗、二抗，充分满足客户多样化实验选型需求。索莱宝拥有成熟专业的抗体制备与质量检测平台，从免疫制备、纯化提纯到实验验证全流程严格筛选评估，全方位保障每一支抗体的性能与品质。

此外，索莱宝已完善一站式技术服务平台，可提供 WB、IHC、IF、IP、流式检测、细胞实验等常规检测，以及多克隆/单克隆抗体定制等个性化定制服务，为科研用户提供全方位解决方案。