- ￥289
- Abcam
- ab6721
- 美国
- 2025年07月16日
- IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
山羊抗兔IgG H&L (HRP)
- 抗体英文名：
Goat Anti-Rabbit IgG H&L (HRP)
- 浓度：
1mg 浓度为 2mg/ml
- 应用范围：
IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr
- 宿主：
Goat
- 供应商：
青岛捷世康生物科技有限公司
- 库存：
10
- 目录编号：
ab6721
- 抗原来源：
Goat
- 保质期：
24个月
- 标记物：
HRP
- 克隆性：
多克隆
- 保存条件：
-20度
- 形态：
液体
- 规格：
100ug
Conjugation: HRP
Host species: Goat
Isotype: IgG
Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr
纯度
Immunogen affinity purified
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
共轭说明
Horseradish Peroxidase (HRP)
克隆
多克隆
同种型
IgG
常规说明
HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
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文献和实验E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
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