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文献和实验Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
Site-specific modification of glycoproteins has wide application in both biochemical and biophysical studies. This method describes the conjugation of synthetic molecules to the N-terminus of a glycoprotein fragment, viz., human
(8 –11 ). The substrate specificity of the enzyme is (M,I,L)XGG-X or (M,I,L)XGX-G (12 ). Alkylating agents and E64 inhibit the protease and virus infection as expected (13 ). Specific inhibitors are not yet available. To date, 17 protease genes
Studies of the Ubiquitin Proteasome System
SDS‐PAGE sample buffer (see recipe for 4×) Anti–specific antigen antibody Anti‐ubiquitin antibody End‐over‐end rotator Boiling water bath
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