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Anti-Alzheimer Precursor Prote

in A4, a.a. 66-81 of APP {N-terminus}, clone 22C11; 50 μg
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  • 询价
  • MAB348
  • 2025年07月16日
  • Immunofluorescence;Western Blotting
  • Mouse
  • Canine;Human;Mouse;Monkey
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      Immunofluorescence;Western Blotting

    • 宿主：

      Mouse

    • 库存：

      大量

    • 适应物种：

      Canine;Human;Mouse;Monkey

    • 抗原来源：

      0

    • 是否单克隆：

      1

    • 规格：

      50 μg

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    Mouse anti-APP (Catalog Number MAB348) Western blot of endogenous APPs from SH-SY5Y conditioned medium.

    Negative Control staining on Alzheimer’s Disease-Hypothalamus. Tissue pretreated with Citrate, pH 6.0. Negative Control Reagent, IHC-Select Detection with HRP-DAB. High mag.

    Amyloid (β-A4-protein, PN: MAB348) staining on Alzheimer’s Disease-Hypothalamus (LEFT image). Tissue pretreated with Citrate, pH 6.0. Monoclonal Ab. diluted to 1:80, IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as cerebral vascular amyloid deposition in the walls of blood vessels (brown). High mag. Negative Control staining on Alzheimer’s Disease-Hypothalamus (RIGHT image). Tissue pretreated with Citrate, pH 6.0. Negative Control Reagent, IHC-Select Detection with HRP-DAB. High mag.

    Species Reactivity Key Applications Host Format Antibody Type
    Ca, H, M, Mk, Po, R, F IF, IH, IH(P), WB Mouse Purified Monoclonal Antibody
    Description:
    Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP {N-terminus}, clone 22C11
    Background Information:
    Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer's Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.
    Alternate Names:
    APP
    Clone:
    22C11
    Specificity:
    Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).
    Key Applications:
    • Immunofluorescence
    • Immunohistochemistry
    • Immunohistochemistry (Paraffin)
    • Western Blotting
    Application Notes:
    Immunofluorescence

    Immunohistochemistry: 5-10 μg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20).

    Western blot: reducing conditions, 10μg/ml.

    Optimal working dilutions and protocols must be determined by the end user.
    Species Reactivity:
    • Canine
    • Human
    • Mouse
    • Monkey
    • Pig
    • Rat
    • Fish
    Isotype:
    IgG1
    Immunogen:
    Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.
    Epitope:
    a.a. 66-81 of APP {N-terminus}
    Molecular Weight:
    110/120/130 kDa
    Control:
    Brain tissue, brain cell lysates
    Presentation:
    Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
    Storage Conditions:
    Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    Gene Symbol:
    • APP
    • PN-II
    • AD1
    • ABETA
    • CTFgamma
    • APPI
    • ABPP
    • A4
    • CVAP
    • PN2
    • PreA4
    • AAA
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    Neurodegenerative Diseases
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Concentration:
    1 mg/mL
    Antibody Type:
    Monoclonal Antibody
    Format:
    Purified
    Host:
    Mouse
    Purification Method:
    Protein A purfied

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein. 详细描述见链接：http://www.millipore.com/catalogue/item/MAB348

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    图标文献和实验
    相关实验

    • 大脑的秘密生活

      [3]Zhang YW，Thompson R，Zhang H，et al．APP processing in Alzheimer's disease．Mol Brain，2011，4:3． [4]Sastre M，Steiner H，Fuchs K，et al．Presenilin-dependent gamma-secretes processing of beta-amyloid precursor protein at a site corresponding to the S3

    • Studies of the Ubiquitin Proteasome System

      and equipment for maintenance of insect cell cultures and generation of recombinant baculovirus (Murphy et al., 2004 ) and batch ( protocol 22 ) or column ( protocol 23 ) purification of APP‐BP1 and Uba

    • Transvascular delivery of small interfering RNA to the centr

      hairpin precursor transcripts into ∼22 nucleotide regulatory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer. In this study, we characterize Drosophila small RNAs that derive from short intronic hairpins termed “mirtrons

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