Anti-Alzheimer Precursor Prote
in, APP 643-695 C-terminal fragment, clone 2.F2.19B4; 100 μL- 询价
- MAB343
- 2025年07月14日
- Western Blotting;Immunohistochemistry (Paraffin)
- Mouse
- Human;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunohistochemistry (Paraffin)
- 宿主：
Mouse
- 库存：
大量
- 抗原来源：
0
- 适应物种：
Human;Rat
- 是否单克隆：
1
- 规格：
100 μL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IH(P), WB | Mouse | Ascites | Monoclonal Antibody |
- Immunohistochemistry (Paraffin)
- Western Blotting
Western blot: 10 μg/mL
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB343
IMMUNOHISTOCHEMISTRY
1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).
2) Wash sections 3 times in distilled water.
3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.
4) Wash sections for 5 min in TBS.
5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.
6) Wash sections 3 times in TBS for 5 min..
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in TBS.
9) Embed sections and examine.
- Human
- Rat
- APP
- PN-II
- AD1
- ABETA
- CTFgamma
- APPI
- ABPP
- A4
- CVAP
- PN2
- PreA4
- AAA
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Carboxyl fragment of APP 643-695 / Jonas. 详细描述见链接：http://www.millipore.com/catalogue/item/MAB343
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