- 询价
- MAB19562
- 2025年07月15日
- ELISA;Western Blotting
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
ELISA;Western Blotting
- 宿主：
Mouse
- 抗原来源：
0
- 适应物种：
Human
- 库存：
大量
- 是否单克隆：
1
- 规格：
100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Laminin γ2, clone D4B5
Background Information:
Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001).
Alternate Names:
Laminin 5
Clone:
D4B5
Specificity:
Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
Key Applications:
- ELISA
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Western blot: 5ug/ml is recommended; dilute antibody in PBS or TBS only, not blocking solutions; blocking with purified compounds is suggested.
Immunohistochemistry of paraffin sections: 5-10ug/ml is suggested. Do not over fix. A light 4% or 2% PFA are best. 4 mm thick paraffin sections are dewaxed, rehydrated and immersed in 0.3% hydrogen peroxide-containing methanol for inactivation of intrinsic peroxidase, followed by treatment with Protease XXIV (Sigma) for 15 min at room temperature (0.01-0.05%). Do not use trypsin. Antibody incubation at 37°C for 1 hour, followed by secondary antibody detection and visualization with DAB (Mizushima et al. 1998). Do not place the monoclonal antibody in serum blocks because serum conatins laminin 5 which will bind and interfere with the antibody. Use BSA or other such media instead.
Strongly positive staining is seen in Basement Membrane of the following tissues: Skin, Esophagus, Lung, and Kidney. Positive staining is also seen in Stomach, Small intestine, Spleen, Thymus, Prostate, and Ovary. Thyroid, Testis, Skeletal and Cardiac muscle were negative for LN5 staining.
Inhibition of alpha2beta1 integrin binding to laminin 5 (40ug/ml) (Decline & Rousselle 2000).
ELISA Optimal working dilutions must be determined by end user.
Immunohistochemistry of paraffin sections: 5-10ug/ml is suggested. Do not over fix. A light 4% or 2% PFA are best. 4 mm thick paraffin sections are dewaxed, rehydrated and immersed in 0.3% hydrogen peroxide-containing methanol for inactivation of intrinsic peroxidase, followed by treatment with Protease XXIV (Sigma) for 15 min at room temperature (0.01-0.05%). Do not use trypsin. Antibody incubation at 37°C for 1 hour, followed by secondary antibody detection and visualization with DAB (Mizushima et al. 1998). Do not place the monoclonal antibody in serum blocks because serum conatins laminin 5 which will bind and interfere with the antibody. Use BSA or other such media instead.
Strongly positive staining is seen in Basement Membrane of the following tissues: Skin, Esophagus, Lung, and Kidney. Positive staining is also seen in Stomach, Small intestine, Spleen, Thymus, Prostate, and Ovary. Thyroid, Testis, Skeletal and Cardiac muscle were negative for LN5 staining.
Inhibition of alpha2beta1 integrin binding to laminin 5 (40ug/ml) (Decline & Rousselle 2000).
ELISA Optimal working dilutions must be determined by end user.
Species Reactivity:
Human
Isotype:
IgG1
Immunogen:
Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein
Molecular Weight:
150 kDa (precursor form) and 105 kDa (mature form)
Control:
Human pancreatic tumor tissue, human breast carcinoma tissue or A-431 whole cell lysate
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- LAMC2
- EBR2
- LAMB2T
- MGC141938
- MGC138491
- LAMNB2
- epiligrin
- BM600-100kDa
- BM600
- kalinin-105kDa
- nicein-100kDa
- EBR2A
- B2T
- CSF
Antibody Category:
Cell Structure
Antibody Sub-Category:
ECM Proteins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Product Family Information
Laminin Antibodies, Proteins & KitsMillipore’s Anti-Laminin antibody demonstrates specificity against Laminin. See below for data, references and related products for Laminin. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein 详细描述见链接：http://www.millipore.com/catalogue/item/MAB19562
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Anti-Laminin gamma2, clone D4B5; 100 μg
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