- 询价
- MAB1924
- 2025年07月16日
- ELISA;Immunoprecipitation
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
ELISA;Immunoprecipitation
- 宿主：
Mouse
- 抗原来源：
0
- 适应物种：
Human
- 库存：
大量
- 是否单克隆：
1
- 规格：
100 μL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IH, IP | Mouse | Ascites | Monoclonal Antibody |
- ELISA
- Immunohistochemistry
- Immunoprecipitation
Immunofluorescence - tissue staining pattern is most consistent with that observed for laminin alpha5 in the mouse(Miner et al., 1997)
Affinity chromatography
Immunoprecipitation
Does not work for Western blotting
Optimal working dilutions must be determined by end user.
- LAMA5
- KIAA1907
- KIAA0533
Product Family Information
Laminin Antibodies, Proteins & KitsMillipore’s Anti-Laminin antibody demonstrates specificity against Laminin. See below for data, references and related products for Laminin. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Purified human laminin 详细描述见链接：http://www.millipore.com/catalogue/item/MAB1924
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文献和实验LABELING ALPHA-ACTININ WITH IATR
.Dialyze overnight against buffer 5 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 12.Determine the concentration of alpha-actinin using Lowry assay.Determine dye/protein molar ratio by diluting conjugate 1:40 (10μl in 400μl
In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
Clone Genes From a Phage Library
for phage. 2.9g NaCl(50 mM), 1g Mg SO4 (10 mM), 25 ml Tris pH 7.5 (50 mM), 50 mg gelatin (100 g/l) in 500 ml-- autoclave. • NaOH for plaque lifts: 0.5M NaOH, 1.5M Na Cl, 1 mM EDTA. • Tris for plaque lifts: 0.5M Tris HCl pH 8, 1.5 M
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