- ￥4500
- 义翘神州
- 40592-V08H8
- 北京
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein
- 保质期：
12个月
- 供应商：
北京义翘神州科技股份有限公司
- 保存条件：
-20℃ to -80℃
- 规格：
100 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombiant Protein (40592-V08H8), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombiant Protein (40592-V08H8) is 25-60 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(G476S)) was expressed with a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Arg319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.57 kDa.
缓冲液Lyophilized from sterile PBS,pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验日前，北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台，针对新型冠状病毒及其变异株，设计了编码新冠病毒刺突蛋白（Spike）受体结构域（RBD）的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗（circRNARBD
, hence lowering the detection limit and preventing faulty results. The following assay protocol was used: 1. Coating of 0.5 µg/ml RBD of SARS-CoV-2 (trenzyme, Germany) in Coating Buffer pH 7.4 2. Aspirate or tap the plate (do not wash) 3. Blocking with 200
三句话读懂一篇 CNS：运动 30 分钟可立即改善抑郁症患者情绪；多喝水或可降低心衰风险
7. Hepatology：肝细胞癌祖细胞到完全建立肝细胞癌过程的潜在机理 肝癌的发生通过肝细胞癌 (HCC) 祖细胞 (HcPCs) 到完全建立的 HCC，其机制不明。 2022 年 3 月 28 日，海军军医大学侯晋及曹雪涛共同通讯在 Hepatology 杂志发表研究论文 Malignant progression of liver cancer progenitors requires KAT7-acetylated and cytoplasm-translocated G-protein GαS
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