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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
Fluorescent Carboxyl Magnetic Particles, High Iron, Yellow, 0.5%w/v,0.46µm, 2mL
- 保质期：
12个月
- 供应商：
东莞汉诺生物
- 保存条件：
2-8度
- 规格：
2ml
| FCFM-2052-2 | Fluorescent Carboxyl Ferromagnetic Particles, Yellow, 1% w/v, 2.0-2.9 um, 2 mL |
| FCFM-40052-2 | Fluorescent Carboxyl FerroMagnetic Particles, Yellow, 1% w/v, 38.0-44.0 um, 2 mL |
| FCFM-4052-2 | Fluorescent Carboxyl FerroMagnetic Particles, Yellow, 1% w/v, 4.0-4.9 um, 2 mL |
| FCFM-4056-2 | Fluorescent Carboxyl FerroMagnetic Particles, Nile Red, 1% w/v, 4.0-4.9 um, 2 mL |
| FCFM-5056-2 | Fluorescent Carboxyl FerroMagnetic Particles, Nile Red, 1% w/v, 5.0-5.9 um, 2 mL |
| FCM-02556-2 | Fluorescent Carboxyl Magnetic Particles, Nile Red, 1% w/v, 0.2-0.39 um, 2 mL |
| FCM-0852-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 0.7-0.9 um, 2 mL |
| FCM-0552-2H | Fluorescent Carboxyl Magnetic Particles, High Iron, Yellow, 0.5% w/v, 0.4-0.69 um, 2 mL |
| FCM-0556-2 | Fluorescent Carboxyl Magnetic Particles, Nile Red, 0.5% w/v, 0.4-0.69 um, 2 mL |
| FCM-0856-2 | Fluorescent Carboxyl Magnetic Particles, Nile Red, 1% w/v, 0.7-0.9 um, 2 mL |
| FCM-100052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 90.0-105 um, 2 mL |
| FCM-10052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 10.0-14.0 um, 2 mL |
| FCM-1045-2 | Fluorescent Carboxyl Magnetic Particles, Light Yellow, 1% w/v, 1.0-1.4 um, 2 mL |
| FCM-1052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 1.0-1.4 um, 2 mL |
| FCM-1056-2 | Fluorescent Carboxyl Magnetic Particles, Nile Red, 1% w/v, 1.0-1.4 um, 2 mL |
| FCM-1058-2 | Fluorescent Carboxyl Magnetic Particles, Pink, 1% w/v, 1.0-1.4 um, 2 mL |
| FCM-1068-2 | Fluorescent Carboxyl Magnetic Particles, Blue, 1% w/v, 1.0-1.4 um, 2 mL |
| FCM-1070-2 | Fluorescent Carboxyl Magnetic Particles, Sky Blue, 1% w/v, 1.0-1.4 um, 2 mL |
| FCM-200052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 180-210 um, 2 mL |
| FCM-20052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 18.0-24.9 um, 2 mL |
| FCM-2042-2 | Fluorescent Carboxyl Magnetic Particles, UV/Light Yellow, 1% w/v, 2.0-2.4 um, 2 mL |
| FCM-2052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 2.0-2.4 um, 2 mL |
| FCM-2058-2 | Fluorescent Carboxyl Magnetic Particles, Pink, 1% w/v, 2.0-2.4 um, 2 mL |
| FCM-2062-2 | Fluorescent Carboxyl Magnetic Particles, Purple, 1% w/v, 2.0-2.4 um, 2 mL |
| FCM-2070-2 | Fluorescent Carboxyl Magnetic Particles, Sky Blue, 1% w/v, 2.0-2.4 um, 2 mL |
| FCM-4052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 4.0-4.9 um, 2 mL |
| FCM-4056-2 | Fluorescent Carboxyl Magnetic Particles, Nile Red, 1% w/v, 4.0-4.9 um, 2 mL |
| FCM-4058-2 | Fluorescent Carboxyl Magnetic Particles, Pink, 1% w/v, 4.0-4.9 um, 2 mL |
| FCM-50052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 44.0-52.9 um, 2 mL |
| FCM-5052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 5.0-5.9 um, 2 mL |
| FCM-7041-2 | Fluorescent Carboxyl Magnetic Particles, UV, 0.1% w/v, 7.0-7.9 um, 2 mL |
| FCM-7067-2 | Fluorescent Carboxyl Magnetic Particles, PAK Blue, 0.1% w/v, 7.0-7.9 um, 2 mL |
| FCM-8052-2 | Fluorescent Carboxyl Magnetic Particles, Yellow, 1% w/v, 8.0-9.9 um, 2 mL |
| FCM-8056-2 | Fluorescent Carboxyl Magnetic Particles, Nile Red, 1% w/v, 8.0-9.9 um, 2 mL |
| FCM-8058-2 | Fluorescent Carboxyl Magnetic Particles, Pink, 1% w/v, 8.0-9.9 um, 2 mL |
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文献和实验被数学证明了，在此我不多说，如果有人不明白我再证明给他看。当然为了养成良好的实验习惯，DMSO的体积还是一致的好。5、检测MTT。还原的MTT在460-630均有较好的吸收，如果你的酶标仪是滤光片，可以选470 nm左右或630 nm左右的滤光片，如果酶标仪有单波长，你可以在检测前扫描一下吸收谱，选用最大细说波长检测就是了，最大波长，大概在550nm附近，必要时加一个参比波长以扣除非特异性吸收。6、吸收值分析。在理想的MTT实验中，如果是细胞抑制实验，不加药物处理组的吸收值应该在0.8-1.2左右
类如下： 直接氧化型： L ＋ O 2 → h v （海萤、海笋） 细菌 ] 过氧化型： L ＋ H 2 O 2 hv （柱头虫、蚯蚓） L ：荧光素， hv ：光量子。 有关萤、海萤和发光细菌的发光机制曾进行了详细研究。在荧光素 - 荧光素酶反应以外的生物发光中，已从腔肠动物（水母、硅角海绵贝）内发现了发光蛋白质。水母的发光蛋白质是通过 Ca 2 而发光的。发光颜色可因动物而异，以蓝、绿、黄色的较多，也有呈红色的。发光
或凋亡细胞?PBS洗涤?制备细胞裂解液?加Ac-DEVD-AMC（caspase-3四肽荧光底物）?37°C反应1h?荧光分光光度计（Polarstar）分析荧光强度（激发光波长380nm，发射光波长为430-460nm）。结果如图10: 3 流式细胞术分析 方法：收获细胞正常或凋亡细胞?PBS洗涤?加Ac-DEVD-AMC?37°C反应1h?UV流式细胞计分析caspase-3阳性细胞数和平均荧光强度。结果如图11：
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