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文献和实验Flow cytometry of yeast cells stained with PI
before computer ENGAGE CELLQUEST SOFTWARE turn on computer double click on "Bob"s FL3h". this loads CellQuest and generates a graph template suitable for collecting data if PI stained yeast an error message comes up, click on "OK
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (dnEBNA-1/Soft)in E.coli BL21 LysS per 0.5L LB+ ampicillin (grow two 0.5L cultures of each) Incubate ~2hrs 37℃250rpm until OD600 = 0.4-0.6 Induce with 5ml 100mM ITPG
In Situ Analysis of Telomerase RNA Gene Expression as a Marker for Tumor Progression
), and the interaction of telomerase with other telomere associated proteins ( 10 , 15 – 20 ; see Fig. 1 ). The use of telomerase as a diagnostic marker and target for cancer therapy relies on the development of reliable assays and technologies to detect telomeres
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