Anti-Calbindin D-28K predilute

Anti-Calbindin D-28K, prediluted

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. The biological roles of Calbindin D28k include, calcium regulation (including calcium transport and uptake, calcification of bone and teeth) and calcium related signalling in neurons and transiently in embryological development. This calbindin also has a role in protecting neurons from apoptotic cell death. Disregulation of this calbindin protein relates to several disease states. Decline in calbindin D28k in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D28k immunoreactivity is specifically absent from neuronal poplulations lost early in ALS, while substantial loss of neurons which do contain the protein occur in patients with Huntington's disease.

Calbindin. Shows no cross-reactivity to calretinin by Western blot. The antibody produces specific staining of cerebellum Purkinje cells, molecular layer dendrites and axonal fibers, stains cell bodies and fibers in neuronal subpopulations.

Immunohistochemistry (Paraffin)

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select® Detection Kits.

Calbindin has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1013-6.

Human

Purified recombinant calbindin.

Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% ProClin 300 as preservative.

Maintain at 2-8oC. Refer to vial for expiration dating.

• CALB1
• D-28K
• CALB
• CAB27
• Calbindin

Neuroscience

Neuronal & Glial Markers

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

Purified

Rabbit

Polyclonal Antibody