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文献和实验Human Pregnane X Receptor (hPXR) Activation Assay in Stable Cell Lines
Analysis of pregnane X receptor (PXR, NR1I2) activation to determine induction of drug metabolizing enzymes and transporters and predict drug-drug interactions (DDIs) is a wildly used technique among in vitro assays. Direct assessment of PXR
A protocol for cleaning and reusing the large 25 x 25 cm plates
of plates to be bleached. 6. Rinse the bleached (disinfected) plates with Millipore NANOpure 0.45um-filtered ddH2O (I use the water from our ddH2O storage/wash tank next to the sink). Rinse the bottom plate first under flowing ddH2O (both sides
solutions for all immunoassay steps. Liquid Plate Sealer® animal-free serves to stabilize antibodies and antigens after coating on polystyrene- or glass surfaces, thus allowing for long-term storage of dried ELISA plates. Stabilization of plates
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