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文献和实验Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
) Pax6 (rabbit polyclonal; Covance, cat. no. PRB278P) Goat‐anti‐rabbit IgG (Jackson ImmunoResearch Laboratories, cat. no. 111‐005‐144
Bromodeoxyuridine Immunohistochemistry
serum and 2 drops anti-mouse IgG-biotinylated Ab in 10 ml PBS, mix (a 1:200 dilution of secondary in block solution). Approximately 40 min. into the incubation, begin to prepare avidin/biotin/peroxidase complex (ABC). Add 1 drop of A to 5 ml PBS, vortex
【转贴】immunofluorescence staining
antibody species, e.g., normal goat serum if using Goat anti-mouse IgG), can also be used.Remove blocking buffer and add Primary Antibody diluted in blocking buffer.Incubate either 30 min at 37 C, 1-2 hours at RT, or overnight at 4C. Remove Primary Antibody
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