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文献和实验Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
of the 85℃ XT buffer and 10 µL of Proteinase K solution (10 mg/mL). Transfer the frozen, ground sample to the warm XT buffer and grind thoroughly until the suspension is evenly dispersed.Transfer homogenate to a labeled 50 ml Oakridge tube. Mix and incubate
Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
1 M KOH Dialysis buffer (see recipe ) Glutathione‐Sepharose immobilized proteins (see recipe ) in A50
Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
Purified protein(s) to be cross‐linked, of a known concentration (6 to 8 nmol minimum) 0.5 M sodium N‐morpholinoethanesulfonate (MES buffer), pH 6.5 (see recipe
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