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文献和实验LINEAGE ANALYSIS USING RETROVIRAL VECTORS
several hundred milliliters of producer cell supernatant. These concentrated stocks are titered and tested for helper virus contamination, and can be stored indefinitely at -80°C or in liquid N2 in small (10- to 50-ml) aliquots. If we anticipate storage of over several months
Propagation, Quantification, Detection, and Storage of West Nile Virus
150 tissue culture flasks 50‐ml disposable polystyrene conical tubes with screw caps (e.g., Falcon) 1.5‐ml or 0.5‐ml O‐ring
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