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文献和实验Phycoerythrin conjugation protocol
, about 2.5 mg/ml PE R-Phycoerythrin, purified (Molecular Probes P-801 in 60% ammonium sulfate) PBS Phosphate buffered saline without Ca2+ /Mg2+ MeOH Methanol
Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
). Transformation by vacuum infiltration Six mg of WS seeds (about 300 seeds) were sown on compost in 40x30 cm sowing flats . The germination was synchronized by cold treatment for 48 h at 40C, and the flats were placed in greenhouse (16 hours day
-based Analysis)Proteinase K (Sigma)Proteinase K buffer (0.05 M tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0)Ampli Taq Gold Buffer (Perkin Elmer)dNTP mixture (Perkin Elmer)PrimersDEPC-treated H2OAmpli Taq Gold Polymerase (Perkin
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