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文献和实验cDNA Synthesis from MOLT-4 Cells
second strand yield. 11. Add 0.5 ml PN buffer from Qiegen Nucleotide Removal Kit to the remaining reaction, and mix it ,load it into the spin column, and follow the pr℃edures comimg with the kit to purify the double strand DNA. 12. Elute the cDNA
along with an appropriate marker and an uncut sample of DNA in question. 3) Purify restricted sample by one of the two methods (A or B) below: A) Qiagen-gel purification kit 1) Electrophorese restricted DNA on 0.8% agarose 2) cut out appropriate bands 3) Follow
marker and an uncut sample of DNA in question. 3) Purify restricted sample by one of the two methods (A or B) below: A) Qiagen-gel purification kit 1) Electrophorese restricted DNA on 0.8% agarose 2) cut out appropriate bands
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