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文献和实验The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
Figure 5. Immunohistochemical staining for viral proteins in infected lungs. C57BL/6J mice were infected intranasally with 2 × 103 FFU of PR8M virus. Serial lung sections were stained with anti‐influenza antibody
of interest. In addition, include a control precipitation with an (IgG) antiserum that is not directed against chromatin proteins. Cell culture from which nuclei are to be extracted (1 x 107 to 1 x 108 cells are required; see Steps 9-16) Cell culture medium
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