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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
.- The antibody reacts with human cytokeratins 5 and 6, and weakly with cytokeratin 4, but not other cytokeratin types.- CK5/6 is normally expressed in the basal cells and some stratum spinosum cells of the stratified squamous epithelium, as well as subpopulations
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Transfection Kit (Clontech) CD34+ cell separation kit (CD34 MicroBead Kit containing anti-human CD34 MicroBeads and Blocking Reagent; Miltenyi Biotec) Cellgro medium (serum-free medium; Cellgenix) DMEM for HSC Fetal calf serum (FCS
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